| Evaluation of fixation time using Diff-Quik for staining of canine mast cell tumor aspirates. | |
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MedLine Citation:
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PMID: 23278320 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: Mast cell tumors are the most common cutaneous tumor in the dog and are often diagnosed via fine-needle aspiration and cytology. Many veterinary practices use Diff-Quik stain for these cases because it is easy to use and provides rapid results. Anecdotal reports suggest that Diff-Quik does not stain mast cell tumor granules well and that increased duration of fixation time can improve staining quality; however, this has not been prospectively evaluated. OBJECTIVES: The aim of this study was to determine if varying fixation time would affect the staining quality of mast cell granules using the Diff-Quik stain. The null hypothesis was that there would be no difference in the staining of the granules based on duration of time in the fixation solution. METHODS: Fine-needle aspirates of cutaneous mast cell tumors were obtained from 21 dogs and distributed on multiple slides. These slides were then stained in Diff-Quik at varying fixation times (ie, 5 seconds, 30 seconds, 1 minute, 2 minutes). One slide was stained with modified Wright stain as a control. Mast cell staining quality was evaluated either by blinded clinicopathologic review (n = 12) or by computer analysis of photomicrographs (n = 6). Results were compared with histopathologic grade. RESULTS: There was no difference in staining quality among groups. CONCLUSIONS: Alteration in fixation time using Diff-Quik does not improve staining characteristics of mast cell tumors. |
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Authors:
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Dorothy E Jackson; Kim A Selting; Melanie S Spoor; Carolyn J Henry; Charles E Wiedmeyer |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-31 |
Journal Detail:
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Title: Veterinary clinical pathology / American Society for Veterinary Clinical Pathology Volume: - ISSN: 1939-165X ISO Abbreviation: Vet Clin Pathol Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2013-1-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9880575 Medline TA: Vet Clin Pathol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 American Society for Veterinary Clinical Pathology. |
Affiliation:
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Departments of Veterinary Medicine and Surgery, College of Veterinary Medicine, Columbia, MO, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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