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Evaluation of fixation time using Diff-Quik for staining of canine mast cell tumor aspirates.
MedLine Citation:
PMID:  23278320     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Mast cell tumors are the most common cutaneous tumor in the dog and are often diagnosed via fine-needle aspiration and cytology. Many veterinary practices use Diff-Quik stain for these cases because it is easy to use and provides rapid results. Anecdotal reports suggest that Diff-Quik does not stain mast cell tumor granules well and that increased duration of fixation time can improve staining quality; however, this has not been prospectively evaluated. OBJECTIVES: The aim of this study was to determine if varying fixation time would affect the staining quality of mast cell granules using the Diff-Quik stain. The null hypothesis was that there would be no difference in the staining of the granules based on duration of time in the fixation solution. METHODS: Fine-needle aspirates of cutaneous mast cell tumors were obtained from 21 dogs and distributed on multiple slides. These slides were then stained in Diff-Quik at varying fixation times (ie, 5 seconds, 30 seconds, 1 minute, 2 minutes). One slide was stained with modified Wright stain as a control. Mast cell staining quality was evaluated either by blinded clinicopathologic review (n = 12) or by computer analysis of photomicrographs (n = 6). Results were compared with histopathologic grade. RESULTS: There was no difference in staining quality among groups. CONCLUSIONS: Alteration in fixation time using Diff-Quik does not improve staining characteristics of mast cell tumors.
Authors:
Dorothy E Jackson; Kim A Selting; Melanie S Spoor; Carolyn J Henry; Charles E Wiedmeyer
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-31
Journal Detail:
Title:  Veterinary clinical pathology / American Society for Veterinary Clinical Pathology     Volume:  -     ISSN:  1939-165X     ISO Abbreviation:  Vet Clin Pathol     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-1-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880575     Medline TA:  Vet Clin Pathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 American Society for Veterinary Clinical Pathology.
Affiliation:
Departments of Veterinary Medicine and Surgery, College of Veterinary Medicine, Columbia, MO, USA.
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