Document Detail

Evaluation of firefly luciferase bioluminescence mediated photodynamic toxicity in cancer cells.
MedLine Citation:
PMID:  16791748     Owner:  NLM     Status:  MEDLINE    
PURPOSE: This work investigated whether fLuc-catalyzed oxidation of D-luciferin generates sufficient light to induce photodynamic toxicity in cancer cells. PROCEDURES: Light emission was assessed via cooled CCD (charge-coupled device) camera. Parental and fLuc expressing cancer cells were exposed to subtoxic concentrations of photosensitizers (Rose Bengal or hypericin) and D-luciferin, sunlight, or lamplight. Toxicity was assessed by MTT assay. RESULTS: fLuc expressing cells emitted up to 500-fold higher levels of photons than parental cell lines. Although exposure to photosensitizer and sunlight reduced survival of various cell lines, survival of fLuc expressing cells incubated with photosensitizer and D-luciferin, or photosensitizer and lamplight, did not differ significantly from parental or untreated cells. CONCLUSIONS: Contesting recent reports, fLuc bioluminescence does not generate sufficient photons to induce Rose Bengal or hypericin photodynamic toxicity in a range of malignant and nonmalignant cell lines, and is not suitable as a generalizable approach to antineoplastic therapy.
Meike L Schipper; Manishkumar R Patel; Sanjiv S Gambhir
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging     Volume:  8     ISSN:  1536-1632     ISO Abbreviation:  Mol Imaging Biol     Publication Date:    2006 Jul-Aug
Date Detail:
Created Date:  2006-06-22     Completed Date:  2006-09-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101125610     Medline TA:  Mol Imaging Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  218-25     Citation Subset:  IM    
Molecular Imaging Program at Stanford (MIPS), Department of Radiology and Bio-X Program, Stanford University, E 150 Clark Center, 318 Campus Drive, Palo Alto, CA 94305-5427, USA.
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MeSH Terms
Antineoplastic Agents / therapeutic use
Luciferases, Firefly / therapeutic use*
Neoplasms / therapy*
Perylene / analogs & derivatives,  therapeutic use
Photochemotherapy / methods*
Photons / therapeutic use
Photosensitizing Agents / therapeutic use*
Rose Bengal / therapeutic use
Tumor Cells, Cultured
Grant Support
5R01CA082214-06/CA/NCI NIH HHS
Reg. No./Substance:
0/Antineoplastic Agents; 0/Photosensitizing Agents; 11121-48-5/Rose Bengal; 198-55-0/Perylene; 548-04-9/hypericin; EC, Firefly

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