Document Detail


Evaluation of extracts of Anthocleista djalonensis, Nauclea latifolia and Uvaria afzalii for activity against bacterial isolates from cases of non-gonococcal urethritis.
MedLine Citation:
PMID:  15099860     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Whole root preparations of three Nigerian medicinal plants, Anthocleista djalonensis, Nauclea latifolia and Uvaria afzalii, used traditionally in combination treatment of sexually transmitted diseases (STD), were extracted by maceration in ethanol, cold and hot water, respectively. The extracts were tested, by agar diffusion and macrobroth dilution methods, for activity against five strains of Staphylococcus aureus and two of Escherichia coli isolated from cases of STD and or urethritis. Four typed bacterial strains, S aureus ATCC 12600, Bacillus subtilis ATCC 6051, Pseudomonas aeruginosa ATCC 10145 and Escherichia coli ATCC 117755 were included as reference organisms. Ethanolic and cold-water extracts of Anthocliesta djalonensis exhibited activity against 9 and 7, respectively, of the 11 test organisms. They were bacteriostatic at minimum inhibitory concentrations (MIC) to the Gram positive strains but bactericidal to the Gram negative strains. Similar crude extracts of Uvaria afzalii showed bactericidal activity restricted to Gram positive (Staphylococcus aureus and Bacillus subtilis) strains. Nauclea latifolia extracts were bacteriostatic to both Gram positive and Gram negative strains. No test strain was susceptible to the hot water extracts of Nauclea latifolia but five and seven strains, were respectively susceptible to similar extracts of Anthocliesta djalonensis and Uvaria afzalii. Of the seven column chromatographic fractions of the ethanolic extract of Uvaria afzalii, F(ua-1) exhibited a bactericidal activity restricted to the Gram negative Escherichia coli strains, which were not susceptible to the crude extract. Fractions, F(ua-2), F(ua-3) and F(ua-4), like the crude extract, were bactericidal against the Gram positive strains only. Thus, partial purification seems to broaden the spectrum of activity and generally improve the potency of Uvaria afzalii. These results apparently justify the use of the three plants in treatment of STD.
Authors:
A S Okoli; C U Iroegbu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of ethnopharmacology     Volume:  92     ISSN:  0378-8741     ISO Abbreviation:  J Ethnopharmacol     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-21     Completed Date:  2004-06-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7903310     Medline TA:  J Ethnopharmacol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  135-44     Citation Subset:  IM    
Copyright Information:
Copyright 2004 Elsevier Ireland Ltd.
Affiliation:
Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Infective Agents / administration & dosage,  pharmacology*,  therapeutic use
Artemia / drug effects
Escherichia coli / classification,  drug effects
Gentianaceae
Gram-Negative Bacteria / drug effects*
Gram-Positive Bacteria / drug effects*
Humans
Lethal Dose 50
Medicine, African Traditional
Medicine, Traditional
Microbial Sensitivity Tests
Nigeria
Phytotherapy*
Plant Extracts / administration & dosage,  pharmacology*,  therapeutic use
Plants, Medicinal*
Rubiaceae
Staphylococcus aureus / classification,  drug effects
Urethritis / microbiology,  prevention & control
Uvaria
Chemical
Reg. No./Substance:
0/Anti-Infective Agents; 0/Plant Extracts

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