| Evaluation of extracellular lipid peroxidation in brain cortex of anaesthetized rats by microdialysis perfusion and high-performance liquid chromatography with fluorimetric detection. | |
| | |
MedLine Citation:
|
PMID: 9210427 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A method for in vivo evaluation of lipid peroxidation in the extracellular space of anaesthetized rat brain cortex was developed. This method involved the use of microdialysis perfusion and high-performance liquid chromatography. The microdialysates, eluted from implanted probes, were reacted with thiobarbituric acid (TBA) prior to analysis by an HPLC system equipped with a fluorescence detector (excitation and emission wavelengths were 515 and 550 nm, respectively). Lipid peroxidation in the extracellular space was evaluated as the concentration of malondialdehyde, a lipid peroxidation end product which reacts with TBA to form a fluorescent conjugate. Significantly increased production of malondialdehyde following hydrogen peroxide perfusion (0.03%, 0.3% at a flow-rate of 1 microl/min) was observed in the brain cortex of anaesthetized rats. |
| | |
Authors:
|
C S Yang; P J Tsai; J P Wu; N N Lin; S T Chou; J S Kuo |
Related Documents
:
|
6450297 - In situ immune complex formation in isolated perfused kidney using homologous antibody. 8790587 - Transglomerular transport of deae dextran in the isolated perfused kidney. 8103107 - The effect of therapeutic doses of paracetamol on liver function in the rat perfused li... 2352157 - Recovery of rat nasal mucosa from the effects of aminopeptidase inhibitors. 12884247 - Effects of glucose on the pharmacokinetics of intravenous chlorzoxazone in rats with ac... 2415837 - Expression of retrovirus-related functions in the methylcholanthrene-induced rat myelog... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of chromatography. B, Biomedical sciences and applications Volume: 693 ISSN: 1387-2273 ISO Abbreviation: J. Chromatogr. B Biomed. Sci. Appl. Publication Date: 1997 Jun |
Date Detail:
|
Created Date: 1997-09-04 Completed Date: 1997-09-04 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9714109 Medline TA: J Chromatogr B Biomed Sci Appl Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 257-63 Citation Subset: IM |
Affiliation:
|
Department of Medical Research, Taichung Veterans General Hospital, Taiwan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anesthesia Animals Cerebral Cortex / metabolism* Chromatography, High Pressure Liquid Extracellular Space / metabolism* Hydrogen Peroxide / pharmacology Lipid Peroxidation* Male Malondialdehyde / analysis Microdialysis Perfusion Rats Rats, Sprague-Dawley Spectrometry, Fluorescence Thiobarbiturates |
| Chemical | |
Reg. No./Substance:
|
0/Thiobarbiturates; 504-17-6/thiobarbituric acid; 542-78-9/Malondialdehyde; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A surface-plasmon-resonance analysis of polylysine interactions with a peptide substrate of protein ...
Next Document: Determination of unchanged [18F]dopamine in human and non-human primate plasma during positron emiss...