Document Detail

Evaluation of ethanol effects on PLC signal transduction pathways using cell lines of neuronal origin.
MedLine Citation:
PMID:  7748314     Owner:  NLM     Status:  MEDLINE    
Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA response after 40 minutes in SH-SY5Y cells, an effect that could be mimicked through protein kinase C stimulation by phorbol esters.
C Alling; O Bergman; C Larsson; P Simonsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Alcohol and alcoholism (Oxford, Oxfordshire). Supplement     Volume:  2     ISSN:  1358-6173     ISO Abbreviation:  Alcohol Alcohol Suppl     Publication Date:  1993  
Date Detail:
Created Date:  1995-06-22     Completed Date:  1995-06-22     Revised Date:  2008-02-26    
Medline Journal Info:
Nlm Unique ID:  8804836     Medline TA:  Alcohol Alcohol Suppl     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  295-9     Citation Subset:  IM    
Department of Psychiatry and Neurochemistry, Lund University, Sweden.
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MeSH Terms
Bradykinin / pharmacology
Carbachol / pharmacology
Cell Line
Enzyme Activation / drug effects
Ethanol / toxicity*
Gene Expression / drug effects
Genes, fos
Neurons / drug effects*,  metabolism
Phosphatidylinositols / metabolism
Protein Kinase C / metabolism
RNA, Messenger / genetics,  metabolism
Signal Transduction / drug effects*
Type C Phospholipases / metabolism*
Reg. No./Substance:
0/Phosphatidylinositols; 0/RNA, Messenger; 51-83-2/Carbachol; 58-82-2/Bradykinin; 64-17-5/Ethanol; EC Kinase C; EC 3.1.4.-/Type C Phospholipases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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