Document Detail


Evaluation of eight commercial kits for Helicobacter pylori IgG antibody detection.
MedLine Citation:
PMID:  8267957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Eight commercial kits and an in-house ELISA for detection of IgG antibodies against Helicobacter pylori were evaluated for their use in diagnosis of H. pylori infection and in epidemiological research: Helico-GTM (Porton-Cambridge), G. A. P. test (Bio-Rad), H. pylori antibodies ELISA (Biometra), Anti-H. pylori IgG EIA (Roche), 2nd generation H. pylori EIA (Roche), Anti-H. pylori MTP-assay (Roche), Pylori stat test kit (Whittaker), Pyloriset latex agglutination kit (Orion), and the in-house ELISA based on heat-stable antigens. Fifty-four patients with dyspepsia (31 H. pylori positive by culture or microscopy) and 68 asymptomatic persons were tested. Sensitivities for the eight kits were 71%, 77%, 90%, 84%, 87%, 94%, 90%, 87%, and 87%, specificities were 74%, 65%, 74%, 74%, 83%, 83%, 70%, 65%, and 65%, respectively. For epidemiological use the estimated seroprevalence varied within approximately 15% in all age groups. Sensitivities and specificities obtained in different studies reveal as great differences in the results with the same kit as between results obtained with different kits in the same study. Kits with the highest sensitivities tend to be the same in all studies. It is therefore more important to test a kit in the population to which it is to be applied than to choose a specific kit.
Authors:
A K Jensen; L P Andersen; C H Wachmann
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  101     ISSN:  0903-4641     ISO Abbreviation:  APMIS     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1994-02-02     Completed Date:  1994-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  795-801     Citation Subset:  IM    
Affiliation:
Department of Clinical Microbiology, University of Copenhagen, Rigshospitalet, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Antibodies, Bacterial / blood*
Child
Enzyme-Linked Immunosorbent Assay / methods
Female
Helicobacter Infections / blood,  diagnosis*,  immunology
Helicobacter pylori* / immunology
Humans
Immunoenzyme Techniques
Immunoglobulin G / analysis,  blood*
Latex Fixation Tests / methods
Male
Middle Aged
Reagent Kits, Diagnostic*
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Immunoglobulin G; 0/Reagent Kits, Diagnostic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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