Document Detail

Evaluation of the efficacy of disinfection procedures against Burkholderia cenocepacia biofilms.
MedLine Citation:
PMID:  18977555     Owner:  NLM     Status:  MEDLINE    
In the present study we evaluated the efficacy of various procedures recommended for the disinfection of respiratory equipment and other materials in cystic fibrosis, using both planktonic and sessile Burkholderia cenocepacia cells. A modified European Suspension Test was performed to determine the effects of the disinfection procedures on planktonic cells. The ability of the treatments to kill sessile cells and to remove biofilm biomass was evaluated using two resazurin-based viability assays and a crystal violet staining on biofilms grown and treated in 96-well microtitre plates. The effect of chlorhexidine and hydrogen peroxide treatments on the viability of sessile B. cenocepacia cells was clearly reduced compared to the effects on planktonic cells. Treatments with low concentrations of sodium hypochlorite (0.05%, 5 min) and acetic acid (1.25%, 15 min) also resulted in insufficient reductions in the number of viable sessile cells. There was no relation between the ability of the disinfectants to remove biofilm biomass and their potential to kill biofilm cells. In conclusion, our study indicates that testing of the efficacy of disinfectants should be performed on both planktonic and sessile cells, with particular attention to their effects on cellular viability.
E Peeters; H J Nelis; T Coenye
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-01
Journal Detail:
Title:  The Journal of hospital infection     Volume:  70     ISSN:  0195-6701     ISO Abbreviation:  J. Hosp. Infect.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-25     Completed Date:  2009-02-09     Revised Date:  2013-05-20    
Medline Journal Info:
Nlm Unique ID:  8007166     Medline TA:  J Hosp Infect     Country:  England    
Other Details:
Languages:  eng     Pagination:  361-8     Citation Subset:  IM    
Laboratory for Pharmaceutical Microbiology, Ghent University, Ghent, Belgium.
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MeSH Terms
Biofilms / drug effects*,  growth & development
Burkholderia cepacia complex / drug effects*,  growth & development
Chlorhexidine / pharmacology
Disinfectants / pharmacology*
Disinfection / methods*
Equipment Contamination / prevention & control
Equipment and Supplies, Hospital
Hydrogen Peroxide / pharmacology
Microbial Sensitivity Tests
Sodium Hypochlorite / pharmacology
Reg. No./Substance:
0/Disinfectants; 55-56-1/Chlorhexidine; 7681-52-9/Sodium Hypochlorite; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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