Document Detail

Evaluation of the effects of the marine toxin okadaic acid by using FETAX assay.
MedLine Citation:
PMID:  17280804     Owner:  NLM     Status:  MEDLINE    
The Frog Embryo Teratogenesis Assay Xenopus (FETAX), is a screening assay using embryos at gastrula stage of the anuran Xenopus laevis to identify substances that may pose a developmental hazard in humans. The FETAX assay evaluates three parameters, i.e. mortality, delayed growth and embryo malformation. In the present investigation, the FETAX protocol was applied to the marine toxin okadaic acid (OA) and the experiments show that OA affects the above parameters in a dose-correlated manner. The morpho-functional modifications induced in embryo organs by OA were also studied. The nervous system, tail skeletal musculature, intestine and kidney appeared particularly damaged, with the former being the most sensitive. On the whole, various advantages emerge from using the FETAX assay: different parameters can be tested simultaneously, the indication of the presence of a potentially dangerous substance is rapid and the assay is a valid alternative to mammalian systems.
Livio Casarini; Antonella Franchini; Davide Malagoli; Enzo Ottaviani
Related Documents :
7082654 - Metabolism of 5,6-epoxyretinoic acid in vivo: isolation of a major intestinal metabolite.
21705224 - Porpoisamides a and b, two novel epimeric cyclic depsipeptides from a florida keys coll...
8442764 - Isoenzyme selective irreversible inhibition of rat and human glutathione s-transferases...
7411544 - Retinoic acid analogues. synthesis and potential as cancer chemopreventive agents.
9678254 - Induction of apoptosis by polyunsaturated fatty acids and its relationship to fatty aci...
19053294 - Gold nanoparticles protected with thiol-derivatized amphiphilic poly(epsilon-caprolacto...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-12
Journal Detail:
Title:  Toxicology letters     Volume:  169     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-26     Completed Date:  2007-05-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  145-51     Citation Subset:  IM    
Department of Animal Biology, University of Modena and Reggio Emilia, via Campi 213/D 41100 Modena, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Abnormalities, Drug-Induced / etiology*
Apoptosis / drug effects
Dose-Response Relationship, Drug
Embryo, Nonmammalian / drug effects*
Intestines / drug effects
Kidney / drug effects
Nervous System Malformations / chemically induced,  embryology
Okadaic Acid / toxicity*
Statistics, Nonparametric
Tail / drug effects
Xenopus laevis / embryology*
Reg. No./Substance:
78111-17-8/Okadaic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Overdose after detoxification: a prospective study.
Next Document:  Geldanamycin augments nitric oxide production and promotes capacitation in boar spermatozoa.