Document Detail


Evaluation of a dry format reagent for CD4+ and CD8+ T-cell enumeration with FACSCount and Guava PCA [corrected].
MedLine Citation:
PMID:  22120802     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: In all CD4+/CD8+ T-cell estimation systems, the reagents used are liquid in nature and have to be transported and stored at 2°-8°C. This causes problems in countries where the ambient temperature is high for most parts of the year or where the laboratories are at remote places.
MATERIALS AND METHODS: We evaluated a dry format of CD4/CD8 reagents from ReaMetrix (Bangalore, India) against the existing liquid reagents from Becton Dickinson (San Jose, CA, USA) and Guava PCA system (Guava Technologies, Hayward, CA, USA). Blood samples collected during March 2009 through May 2009 from 102 HIV-infected individuals and 31 normal healthy individuals in a tertiary care centre in India (south) were tested by Guava EasyCD4 System (PCA) and FACSCount using the respective reagents and the corresponding ReaMetrix reagents. Results: Overall, the correlation (r) of the new Rea T Count and FACSCount reagents for the CD4+ T-cell estimation was 0.98, while with ReaPan 3 4 G reagent in the Guava PCA system with the Guava reagent was 0.97. The mean bias for CD4+ T-cell measurements between Rea T count and BD reagent was -6 cells/ml, while the same with ReaPan 3 4 G reagent in the Guava PCA system was 78 cells/ml. The mean bias for the Rea T count and the ReaPan 3 4 G reagent tested in the FACSCount and Guava PCA system was 17 cells.
CONCLUSIONS: The dry reagents were found to be reliable and cheaper compared to the existing liquid reagents. This allows the transportation of reagents in the absence of cold chain and will facilitate a more user-friendly CD4+ T-cell testing system.
Authors:
J Sachithanandham; K Solomon; S Prasannakumar; G Nithyandham; R Kannangai
Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Indian journal of medical microbiology     Volume:  29     ISSN:  1998-3646     ISO Abbreviation:  Indian J Med Microbiol     Publication Date:    2011 Oct-Dec
Date Detail:
Created Date:  2011-11-28     Completed Date:  2012-03-15     Revised Date:  2012-04-03    
Medline Journal Info:
Nlm Unique ID:  8700903     Medline TA:  Indian J Med Microbiol     Country:  India    
Other Details:
Languages:  eng     Pagination:  401-5     Citation Subset:  IM    
Affiliation:
Department of Clinical Virology, Christian Medical College, Vellore 632 004, India.
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MeSH Terms
Descriptor/Qualifier:
Adult
CD4-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / immunology
Female
Flow Cytometry / methods*
HIV Infections / immunology
Humans
India
Lymphocyte Count / methods*
Male
Polymerase Chain Reaction / methods*
Comments/Corrections
Erratum In:
Indian J Med Microbiol. 2012 Jan-Mar;30(1):111

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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