Document Detail

Evaluation of a direct assay for atrial natriuretic peptide.
MedLine Citation:
PMID:  2522028     Owner:  NLM     Status:  MEDLINE    
A direct radioimmunoassay for the assessment of human atrial natriuretic peptide (ANP) in plasma, using a highly specific antibody and a well-defined monoiodotyrosyl-tracer was developed and evaluated by concurrent application of an extraction method. Sensitivity was 13.4 pg/ml; intra- and interassay variations were 3.1 and 5.5%, respectively; recovery averaged 99%; normal values ranged from 15-111 pg/ml (mean +/- SD = 59 +/- 25 pg/ml, n = 41). The results, including the effect of exercise, of the two methods correlated well. Pooling ANP values in normal subjects and patients with congestive heart failure gave a good correlation (p less than 0.01). However, due to processes in unextracted plasma, in the lower range the results from the direct method were erratic. Velocity and duration of centrifugation changed the number of platelets, but no effect on ANP levels, whether assessed by the direct or by the extraction method, was observed. Although the direct method is considerably less laborious than the extracted method its lack of reliability disqualifies it for most purposes.
A C Tan; F M Rosmalen; J A Hofman; P W Kloppenborg; T J Benraad
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  179     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-04-19     Completed Date:  1989-04-19     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-11     Citation Subset:  IM    
Department of Experimental and Chemical Endocrinology, St. Radboud Hospital, Nijmegen, The Netherlands.
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MeSH Terms
Atrial Natriuretic Factor / analysis*
Reg. No./Substance:
85637-73-6/Atrial Natriuretic Factor

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