| Evaluation of a direct assay for atrial natriuretic peptide. | |
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MedLine Citation:
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PMID: 2522028 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A direct radioimmunoassay for the assessment of human atrial natriuretic peptide (ANP) in plasma, using a highly specific antibody and a well-defined monoiodotyrosyl-tracer was developed and evaluated by concurrent application of an extraction method. Sensitivity was 13.4 pg/ml; intra- and interassay variations were 3.1 and 5.5%, respectively; recovery averaged 99%; normal values ranged from 15-111 pg/ml (mean +/- SD = 59 +/- 25 pg/ml, n = 41). The results, including the effect of exercise, of the two methods correlated well. Pooling ANP values in normal subjects and patients with congestive heart failure gave a good correlation (p less than 0.01). However, due to processes in unextracted plasma, in the lower range the results from the direct method were erratic. Velocity and duration of centrifugation changed the number of platelets, but no effect on ANP levels, whether assessed by the direct or by the extraction method, was observed. Although the direct method is considerably less laborious than the extracted method its lack of reliability disqualifies it for most purposes. |
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Authors:
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A C Tan; F M Rosmalen; J A Hofman; P W Kloppenborg; T J Benraad |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 179 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1989 Jan |
Date Detail:
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Created Date: 1989-04-19 Completed Date: 1989-04-19 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1-11 Citation Subset: IM |
Affiliation:
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Department of Experimental and Chemical Endocrinology, St. Radboud Hospital, Nijmegen, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Atrial Natriuretic Factor / analysis* Centrifugation Exercise Female Humans Male Radioimmunoassay |
| Chemical | |
Reg. No./Substance:
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85637-73-6/Atrial Natriuretic Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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