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Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research.
MedLine Citation:
PMID:  22287976     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The aim of the current study was to evaluate primary (human bronchial epithelial cells, HBEC) and non-primary (Calu-3, BEAS-2B, BEAS-2B R1) bronchial epithelial cell culture systems as air-liquid interface- (ALI-) differentiated models for asthma research. Ability to differentiate into goblet (MUC5AC+) and ciliated (β-Tubulin IV+) cells was evaluated by confocal imaging and qPCR. Expression of tight junction/adhesion proteins (ZO-1, E-Cadherin) and development of transepithelial electrical resistance (TEER) were assessed. Primary cells showed localised MUC5AC, β-Tubulin IV, ZO-1, and E-Cadherin and developed TEER with, however, a large degree of inter- and intradonor variation. Calu-3 cells developed a more reproducible TEER and a phenotype similar to primary cells although with diffuse β-Tubulin IV staining. BEAS-2B cells did not differentiate or develop tight junctions. These data highlight the challenges in working with primary cell models and the need for careful characterisation and selection of systems to answer specific research questions.
Authors:
Ceri E Stewart; Elizabeth E Torr; Nur H Mohd Jamili; Cynthia Bosquillon; Ian Sayers
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Publication Detail:
Type:  Journal Article     Date:  2012-01-11
Journal Detail:
Title:  Journal of allergy     Volume:  2012     ISSN:  1687-9791     ISO Abbreviation:  J Allergy (Cairo)     Publication Date:  2012  
Date Detail:
Created Date:  2012-01-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101535174     Medline TA:  J Allergy (Cairo)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  943982     Citation Subset:  -    
Affiliation:
Division of Therapeutics and Molecular Medicine, Nottingham Respiratory Biomedical Research Unit, University Hospital of Nottingham, Nottingham NG7 2UH, UK.
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