| Evaluation of different serological tests for the detection of antibodies against highly pathogenic avian influenza in experimentally infected ostriches (Struthio camelus). | |
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MedLine Citation:
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PMID: 20390531 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study we collected 177 serum samples from ostriches (Struthio camelus) infected experimentally with A/ostrich/South Africa/Middleton/2004 (H5N2) highly pathogenic avian influenza virus. We tested these samples using the haemagglutination inhibition (HI) test, the agar gel immunodiffusion test and three enzyme-linked immunosorbent assay kits. We considered the HI test, with homologous antigen and including pre-treatment of sera with 10% chicken red blood cells, as the gold standard. Detectable specific antibodies appeared on day 7 post-infection and persisted until the termination of the experiment. The relative sensitivity and specificity of the tests under evaluation and Cohen's K value were calculated. The results reported herein could be of assistance to decision-makers in drafting guidelines for the definition of the health status of ostriches and for trade purposes. |
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Authors:
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Anna Toffan; Adriaan Olivier; Marzia Mancin; Valentina Tuttoilmondo; Daniele Facco; Ilaria Capua; Calogero Terregino |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Avian pathology : journal of the W.V.P.A Volume: 39 ISSN: 1465-3338 ISO Abbreviation: Avian Pathol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-07-20 Revised Date: 2010-09-30 |
Medline Journal Info:
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Nlm Unique ID: 8210638 Medline TA: Avian Pathol Country: England |
Other Details:
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Languages: eng Pagination: 11-5 Citation Subset: IM |
Affiliation:
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OIE, FAO and National Reference Laboratory for Avian Influenza and Newcastle Disease-Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Padova, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Viral / blood*, immunology Enzyme-Linked Immunosorbent Assay / methods Hemagglutination Inhibition Tests / methods Immunodiffusion / methods Influenza A Virus, H5N2 Subtype / immunology*, isolation & purification Influenza in Birds / blood, diagnosis*, immunology, virology Sensitivity and Specificity Struthioniformes* / immunology, virology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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