| Evaluation of different models of experimentally induced liver cirrhosis for MRI research with correlation to histopathologic findings. | |
| | |
MedLine Citation:
|
PMID: 10226849 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
RATIONALE AND OBJECTIVES: Three models of experimentally induced liver cirrhosis were evaluated for MRI research on chronic liver disease. The influence of different histopathologic changes in liver fibrosis and cirrhosis on relaxation times and signal intensities was studied in vitro and in vivo. METHODS: Liver fibrosis and cirrhosis in rats was induced by oral or subcutaneous administration of carbon tetrachloride (CCl4) or by thioacetamide (TAA) in drinking water. On histology, the degree of liver fibrosis and cirrhosis, fatty infiltration, iron accumulation, and inflammatory changes were measured semiquantitatively. The amount of connective tissue was quantitatively determined by morphometry. The results were correlated with T1 and T2 relaxation times and signal intensities of the liver studied in vitro by relaxometry and in vivo by MRI. RESULTS: In both groups with CCl4 administration, histology revealed different degrees of liver fibrosis and cirrhosis. Subcutaneous injection of CCl4 also resulted in increased fatty infiltration. On the contrary, TAA produced complete liver cirrhosis in all animals. Overall, there was a good correlation between the liver T2 relaxation time and the amount of connective tissue in liver fibrosis and cirrhosis. However, the degree of liver fibrosis and cirrhosis was also strongly correlated with the degree of inflammatory changes. In the group with CCl4 administration, there was a good correlation between the fatty infiltration and the T1 relaxation time, as well as with the liver signal intensity on the T1-weighted gradient echo sequence. An increased iron accumulation was also correlated with the degree of liver fibrosis/cirrhosis; however, there was no significant influence of the iron on relaxation times or signal intensities. CONCLUSIONS: The TAA model is easier to perform and more reliable in liver cirrhosis induction than the CCl4 models. Although there is a positive correlation between the T2 relaxation times and the degree of liver fibrosis/cirrhosis, this probably results from the associated inflammatory changes and is not caused by the increased amount of connective tissue. |
| | |
Authors:
|
B Kreft; F Dombrowski; W Block; R Bachmann; U Pfeifer; H Schild |
Related Documents
:
|
20132999 - Normal to increased thrombin generation in patients undergoing liver transplantation de... 20807229 - The effects of in vitro hemodilution with 6% hydroxyethyl starch (hes) (130/0.4) soluti... 11468249 - Heterozygous m3mmalton alpha1-antitrypsin deficiency associated with end-stage liver di... 3066109 - Fatal yersinia enterocolitica septicemia complicated by unique hemosiderosis. a case re... 9820409 - Primary amyloidosis with spontaneous splenic rupture, cholestasis, and liver failure tr... 17357379 - Renal and liver transplantation for toxin-induced organ failure. 1708009 - Anemia and angiotensin-converting enzyme inhibition in renal transplant recipients. 8126839 - Intraperitoneal drainage of recurrent lymphoceles using an internalized tenckhoff cathe... 3243179 - Famotidine-associated mental confusion in elderly patients. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Investigative radiology Volume: 34 ISSN: 0020-9996 ISO Abbreviation: Invest Radiol Publication Date: 1999 May |
Date Detail:
|
Created Date: 1999-07-01 Completed Date: 1999-07-01 Revised Date: 2009-11-11 |
Medline Journal Info:
|
Nlm Unique ID: 0045377 Medline TA: Invest Radiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 360-6 Citation Subset: IM |
Affiliation:
|
Department of Radiology, University of Bonn, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Carbon Tetrachloride / toxicity Carcinogens / toxicity Chronic Disease Follow-Up Studies Liver / pathology* Liver Cirrhosis, Experimental / chemically induced, diagnosis* Magnetic Resonance Imaging* Rats Rats, Sprague-Dawley Severity of Illness Index Thioacetamide / toxicity |
| Chemical | |
Reg. No./Substance:
|
0/Carcinogens; 56-23-5/Carbon Tetrachloride; 62-55-5/Thioacetamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Influence of aging on gastrointestinal transit time. An ultrasonographic and radiologic study.
Next Document: Short-term effects of a new intravascular nitinol stent in canine arteries.