Document Detail


Evaluation of cytotoxicity of two root canal filling materials by MTT assay.
MedLine Citation:
PMID:  20377560     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to assess the cytotoxicity of two root filling materials GuttaFlow (GF) and gutta-percha (GP) on mouse fibroblasts cell line L-929. In this study there were four groups: GP and GF were considered as study groups and the other two were negative control groups. GP and GF were prepared according to manufacturer's instruction. L-929 fibroblast cells of mouse were passaged with trypsin (Merck, Germany) after elimination of freeze phase. Adequate trypsin was added to cells and they were prepared with 95% of cell vitality. After 24 h, 150,000 cells were put in each well. The cell and dimethyl methacrylate were used as negative and positive controls. Ten specimens from each group were brought into contact with the culture medium and were incubated under sterilised conditions 24 h later. The cytotoxicity of all samples was assessed by dimethylthiazol diphenyltetrazolium bromide test after 1 h, 24 h and 72 h. The results showed that cytotoxicity of GF was less than GP when assessed at 24 h and 72 h, but there was no significant difference at 1 h. In GF, the most and least cytotoxicity were observed at 24 h and 72 h while cytotoxicity of GP increased with time.
Authors:
Hengameh Ashraf; Ali Taherian; Alireza N Kerdar
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Australian endodontic journal : the journal of the Australian Society of Endodontology Inc     Volume:  36     ISSN:  1747-4477     ISO Abbreviation:  Aust Endod J     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-09     Completed Date:  2010-07-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888004     Medline TA:  Aust Endod J     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  24-8     Citation Subset:  D    
Affiliation:
Department of Endodontics, School of Dentistry, Shaheed Beheshti Medical University, Evin, Tehran, Iran. he_ashraaf@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Dimethylpolysiloxanes / toxicity*
Drug Combinations
Fibroblasts / drug effects*
Gutta-Percha / toxicity*
L Cells (Cell Line)
Materials Testing
Mice
Root Canal Filling Materials / toxicity*
Tetrazolium Salts / metabolism
Thiazoles / metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Dimethylpolysiloxanes; 0/Drug Combinations; 0/GuttaFlow; 0/Root Canal Filling Materials; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue; 9000-32-2/Gutta-Percha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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