Document Detail


Evaluation of cytotoxic effects induced by bcl-2 and bcl-xL antisense-oligodeoxynucleotides in normal urothelium and transitional cell carcinoma.
MedLine Citation:
PMID:  19020723     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report on cytotoxic effects of bcl-2 and bcl-xL antisense-oligodeoxynucleotides (AS-ODNs) in benign urothelial and transitional cell carcinoma (TCC) cell lines. The benign urothelial cell line (UROtsa) and four TCC cell lines (UM-UC-3, RT 112 , HT 1197 and T 24/83) were incubated with bcl-2 and bcl-xL AS-ODNs and cell mortality rates were assessed. Bcl-2 and bcl-xL AS-ODN treatment resulted in low toxicity in UROtsa cells (6% and 10% cell mortality, respectively). After bcl-2 AS-ODN treatment, cell mortality rates in TCC cell lines were significantly higher than in UROtsa cells (mean values: 33% vs. 6%, respectively). Bcl-2 AS-ODN treatment also caused significantly higher cell death rates in the majority of TCC cell lines when compared to bcl-xL-AS-ODN therapy (mean values: 33% vs. 11%, respectively). In conclusion, bcl-2 AS-ODNs show significantly higher cell mortality in TCC cells, whereas toxic effects on normal urothelium seem to be minor. Our results suggest favourable characteristics for the clinical application of AS-ODN in intravesical chemotherapy of TCC.
Authors:
Ute Gabriel; Christian Bolenz; Andreas Becker; Axel Schaaf; Annette Steidler; Lutz Trojan; Christel Weiss; Maurice Stephan Michel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  20     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-02-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1419-23     Citation Subset:  IM    
Affiliation:
Department of Urology, Mannheim Medical Center, University of Heidelberg, 68167 Mannheim, Germany. ute.gabriel@uro.ma.uni-heidelberg.de
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Transitional Cell / metabolism*
Cell Line, Tumor
Cell Survival
Gene Expression Regulation, Neoplastic
Gene Therapy / methods
Humans
Immunohistochemistry / methods
Oligonucleotides, Antisense / metabolism*
Proto-Oncogene Proteins c-bcl-2 / genetics*,  metabolism
Transcription, Genetic
Urinary Bladder Neoplasms / metabolism*
Urothelium / drug effects,  pathology*
bcl-X Protein / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Oligonucleotides, Antisense; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-X Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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