Document Detail

Evaluation of cytoprotective drugs for liver preservation by pyridine nucleotide fluorometry.
MedLine Citation:
PMID:  3291169     Owner:  NLM     Status:  MEDLINE    
The cytoprotective effects of membrane-stabilizing drugs, such as chlorpromazine, allopurinol, dibucaine, phenoxybenzamine, and OP41483 (prostacyclin analogue), administered to perfusate and preservation medium were studied in rat liver, after 24 hours' preservation, by assessment of pyridine nucleotide fluorescence. On the fluorometric trace curve, amplitude (RxA) and velocity (RxV) from oxidation to reduction were determined. Percent decrease of RxA (%RxA) and that of RxV (%RxV) after 24 hours' preservation were calculated. At the end of preservation, the concentration of total adenine nucleotides of the liver, hepatic adenylate energy charge, and prepared mitochondrial oxidative phosphorylative activity were also measured. In the groups given phenoxybenzamine, dibucaine, and allopurinol, there was no significant difference among these parameters. In the chlorpromazine group, energy charge and %RxV were higher than in the drug-free group (p less than 0.05). In the OP41483 group, both energy charge and phosphorylation rate were significantly higher (p less than 0.05) and %RxV was significantly high (p less than 0.01) at concentrations of more than 3 nmol/L, compared with the values for those without drugs. These results suggest that the Redoximeter can provide accurate information on the effectiveness of cytoprotective drugs. It is also suggested that OP41483 has potential application for maintaining graft viability for human liver transplantation.
N Ozaki; Y Tokunage; S Wakashiro; I Ikai; T Morimoto; Y Shimahara; Y Kamiyama; Y Yamaoka; K Ozawa; Y Nakase
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Surgery     Volume:  104     ISSN:  0039-6060     ISO Abbreviation:  Surgery     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-08-04     Completed Date:  1988-08-04     Revised Date:  2009-08-14    
Medline Journal Info:
Nlm Unique ID:  0417347     Medline TA:  Surgery     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  98-103     Citation Subset:  AIM; IM    
Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
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MeSH Terms
Allopurinol / pharmacology
Cell Membrane / drug effects*
Chlorpromazine / pharmacology
Dibucaine / pharmacology
Epoprostenol / pharmacology
Liver / analysis,  drug effects*
Mitochondria, Liver / metabolism
Organ Preservation*
Phenoxybenzamine / pharmacology
Prostaglandins, Synthetic / pharmacology
Pyrimidine Nucleotides
Rats, Inbred Strains
Reg. No./Substance:
0/Prostaglandins, Synthetic; 0/Pyrimidine Nucleotides; 315-30-0/Allopurinol; 35121-78-9/Epoprostenol; 50-53-3/Chlorpromazine; 59-96-1/Phenoxybenzamine; 83997-19-7/ataprost; 85-79-0/Dibucaine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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