Document Detail


Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa.
MedLine Citation:
PMID:  3902897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One hundred and three gastroscopic biopsies from 80 patients were cultured for Campylobacter pyloridis and studied histologically. Active chronic gastritis, as shown by the presence of polymorphonuclear leucocytes, was diagnosed in 51 biopsies and C pyloridis was found in 47. Sixteen gastric biopsies showed normal histology (no inflammation); C pyloridis was detected in only one of these, and a second biopsy taken from this patient at the same time showed active gastritis. Biopsies could be kept at 4 degrees C for five hours without loss of viability of C pyloridis. An inoculum made by grinding the biopsy in a ground glass grinder consistently gave a much heavier growth of C pyloridis than one made by mincing the specimen. The campylobacter supplement ferrous sulphate, sodium metabisulphite, sodium pyruvate (FBP) (Oxoid) was inhibitory for some isolates; the inhibitory component was found to be sodium metabisulphite. Contaminants, but not C pyloridis, were inhibited by the incorporation of vancomycin 6 mg/l, nalidixic acid 20 mg/l, and amphotericin 2 mg/l, but higher concentrations inhibited C pyloridis. Undried plates kept in a plastic container at room temperature for up to two weeks were as satisfactory as freshly poured plates for the isolation of C pyloridis.
Authors:
C S Goodwin; E D Blincow; J R Warren; T E Waters; C R Sanderson; L Easton
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  38     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1985-12-03     Completed Date:  1985-12-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1127-31     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Atmospheric Pressure
Bacteriological Techniques*
Campylobacter / drug effects,  isolation & purification*
Culture Media
Esophagus / microbiology
Female
Gastric Mucosa / microbiology*
Gastroscopy
Humans
Male
Middle Aged
Nalidixic Acid / pharmacology
Vancomycin / pharmacology
Chemical
Reg. No./Substance:
0/Culture Media; 1404-90-6/Vancomycin; 389-08-2/Nalidixic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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