Document Detail

Evaluation of a cow-side immunoassay kit for assessing IgG concentration in colostrum.
MedLine Citation:
PMID:  16013548     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine sensitivity and specificity of a cow-side immunoassay kit for assessing IgG concentration in colostrum. DESIGN: Prospective study. ANIMALS: 76 dairy and 11 beef cows of various parities. PROCEDURE: Colostrum from first, second, and third milkings and milk samples were collected, and IgG concentration was determined by means of radial immunodiffusion. The immunoassay was performed according to the manufacturer's instructions, and sensitivity and specificity were calculated by comparing results of the immunoassay (positive vs negative) with results of immunodiffusion (< 50 g/L vs > or = 50 g/L). RESULTS: 135 colostrum or milk samples were collected. Mean +/- SD colostral IgG concentrations, determined by means of radial immunodiffusion for dairy and beef cows were 65.4 +/- 51.4 g/L and 114.8 +/- 42.7 g/L, respectively. Mean IgG concentrations for first-, second-, and third-milking colostrum samples and for milk samples were 92 +/- 49.0 g/L, 74.6 +/- 45.1 g/L, 47.5 +/- 32 g/L, and 6.8 +/- 3.8 g/L, respectively. Sensitivity of the immunoassay (ie, percentage of samples with IgG concentration < 50 g/L with a positive immunoassay result) was 93%, and specificity (ie, percentage of samples with IgG concentration > or = 50 g/L with a negative immunoassay result) was 76%. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that the immunoassay kit was an acceptable cow-side test to identify colostrum samples with IgG concentrations < 50 g/L. The immunoassay kit should be useful in screening colostrum for adequate IgG concentration before feeding to calves or storage.
Munashe Chigerwe; Maisie E Dawes; Jeff W Tyler; John R Middleton; Malantus P Moore; Dusty M Nagy
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American Veterinary Medical Association     Volume:  227     ISSN:  0003-1488     ISO Abbreviation:  J. Am. Vet. Med. Assoc.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-14     Completed Date:  2005-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503067     Medline TA:  J Am Vet Med Assoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  129-31     Citation Subset:  IM    
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA.
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MeSH Terms
Cattle / immunology*,  physiology
Colostrum / immunology*
Immunoassay / methods,  standards,  veterinary*
Immunodiffusion / methods,  veterinary
Immunoglobulin G / analysis*
Lactation / immunology
Milk / immunology
Prospective Studies
Reagent Kits, Diagnostic
Sensitivity and Specificity
Reg. No./Substance:
0/Immunoglobulin G; 0/Reagent Kits, Diagnostic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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