| Evaluation of a cow-side immunoassay kit for assessing IgG concentration in colostrum. | |
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MedLine Citation:
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PMID: 16013548 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine sensitivity and specificity of a cow-side immunoassay kit for assessing IgG concentration in colostrum. DESIGN: Prospective study. ANIMALS: 76 dairy and 11 beef cows of various parities. PROCEDURE: Colostrum from first, second, and third milkings and milk samples were collected, and IgG concentration was determined by means of radial immunodiffusion. The immunoassay was performed according to the manufacturer's instructions, and sensitivity and specificity were calculated by comparing results of the immunoassay (positive vs negative) with results of immunodiffusion (< 50 g/L vs > or = 50 g/L). RESULTS: 135 colostrum or milk samples were collected. Mean +/- SD colostral IgG concentrations, determined by means of radial immunodiffusion for dairy and beef cows were 65.4 +/- 51.4 g/L and 114.8 +/- 42.7 g/L, respectively. Mean IgG concentrations for first-, second-, and third-milking colostrum samples and for milk samples were 92 +/- 49.0 g/L, 74.6 +/- 45.1 g/L, 47.5 +/- 32 g/L, and 6.8 +/- 3.8 g/L, respectively. Sensitivity of the immunoassay (ie, percentage of samples with IgG concentration < 50 g/L with a positive immunoassay result) was 93%, and specificity (ie, percentage of samples with IgG concentration > or = 50 g/L with a negative immunoassay result) was 76%. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that the immunoassay kit was an acceptable cow-side test to identify colostrum samples with IgG concentrations < 50 g/L. The immunoassay kit should be useful in screening colostrum for adequate IgG concentration before feeding to calves or storage. |
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Authors:
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Munashe Chigerwe; Maisie E Dawes; Jeff W Tyler; John R Middleton; Malantus P Moore; Dusty M Nagy |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the American Veterinary Medical Association Volume: 227 ISSN: 0003-1488 ISO Abbreviation: J. Am. Vet. Med. Assoc. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-07-14 Completed Date: 2005-08-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7503067 Medline TA: J Am Vet Med Assoc Country: United States |
Other Details:
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Languages: eng Pagination: 129-31 Citation Subset: IM |
Affiliation:
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Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle / immunology*, physiology Colostrum / immunology* Female Immunoassay / methods, standards, veterinary* Immunodiffusion / methods, veterinary Immunoglobulin G / analysis* Lactation / immunology Milk / immunology Prospective Studies Reagent Kits, Diagnostic Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin G; 0/Reagent Kits, Diagnostic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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