| Evaluation of a commercially available radioimmunoassay and species-specific ELISAs for measurement of high concentrations of insulin in equine serum. | |
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MedLine Citation:
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PMID: 23013186 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objective-To evaluate a human radioimmunoassay (RIA) and equine and high-range porcine (hrp) species-specific ELISAs for the measurement of high serum insulin concentrations in ponies. Samples-Serum samples from 12 healthy nonobese ponies (7 clinically normal and 5 laminitis prone; 13 to 26 years of age; 11 mares and 1 gelding) before and after glucose, insulin, and dexamethasone administration. Procedures-Intra-and interassay repeatability, freeze-thaw stability, dilutional parallelism, and assay agreement were assessed. Results-Assay detection limits were as follows: RIA, < 389 μU/mL; equine ELISA, < 175 μU/mL; and hrp ELISA, 293 to 8,775 μU/mL. Mean ± SD intra- and interassay repeatability were respectively as follows: RIA, 6.5 ± 5.1 % and 74 ± 3.4%; equine ELISA, 10.6 ± 11.0% and 9.0 ± 4.6%; and hrp ELISA, 19.9 ± 172% and 173 ± 16.6%. Freezing and thawing affected measured concentrations. Dilutional parallelism in the RIA was only evident when insulin-depleted equine serum was used as a diluent (percentage recovery, 95.7 ± 274%); in the ELISAs, dilutional parallelism was observed when a zero calibrator was used. Agreement between RIA and equine ELISA results was good for samples containing concentrations < 175 μU of insulin/mL (bias, -18.5 ± 25.5 μU/mL; higher in RIA). At higher concentrations, assay agreement was poor between RIA and equine ELISA results (bias, -185.3 ± 98.7 μU/mL) and between RIA and hrp ELISA results (bias, 25.3 ± 183.0 μU/mL). Conclusions and Clinical Relevance-Agreement among results of the 3 assays was variable, and dilutional parallelism was only evident with the RIA when insulin-depleted equine serum was tested. Caution is recommended when evaluating high insulin concentrations measured with the RIA or ELISAs. |
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Authors:
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Kate E Borer-Weir; Simon R Bailey; Nicola J Menzies-Gow; Patricia A Harris; Jonathan Elliott |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of veterinary research Volume: 73 ISSN: 1943-5681 ISO Abbreviation: Am. J. Vet. Res. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-09-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375011 Medline TA: Am J Vet Res Country: United States |
Other Details:
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Languages: eng Pagination: 1596-602 Citation Subset: IM |
Affiliation:
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Department of Veterinary Clinical Sciences, Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, AL9 7TA, England. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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