Document Detail


Evaluation of a commercially available radioimmunoassay and species-specific ELISAs for measurement of high concentrations of insulin in equine serum.
MedLine Citation:
PMID:  23013186     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Objective-To evaluate a human radioimmunoassay (RIA) and equine and high-range porcine (hrp) species-specific ELISAs for the measurement of high serum insulin concentrations in ponies. Samples-Serum samples from 12 healthy nonobese ponies (7 clinically normal and 5 laminitis prone; 13 to 26 years of age; 11 mares and 1 gelding) before and after glucose, insulin, and dexamethasone administration. Procedures-Intra-and interassay repeatability, freeze-thaw stability, dilutional parallelism, and assay agreement were assessed. Results-Assay detection limits were as follows: RIA, < 389 μU/mL; equine ELISA, < 175 μU/mL; and hrp ELISA, 293 to 8,775 μU/mL. Mean ± SD intra- and interassay repeatability were respectively as follows: RIA, 6.5 ± 5.1 % and 74 ± 3.4%; equine ELISA, 10.6 ± 11.0% and 9.0 ± 4.6%; and hrp ELISA, 19.9 ± 172% and 173 ± 16.6%. Freezing and thawing affected measured concentrations. Dilutional parallelism in the RIA was only evident when insulin-depleted equine serum was used as a diluent (percentage recovery, 95.7 ± 274%); in the ELISAs, dilutional parallelism was observed when a zero calibrator was used. Agreement between RIA and equine ELISA results was good for samples containing concentrations < 175 μU of insulin/mL (bias, -18.5 ± 25.5 μU/mL; higher in RIA). At higher concentrations, assay agreement was poor between RIA and equine ELISA results (bias, -185.3 ± 98.7 μU/mL) and between RIA and hrp ELISA results (bias, 25.3 ± 183.0 μU/mL). Conclusions and Clinical Relevance-Agreement among results of the 3 assays was variable, and dilutional parallelism was only evident with the RIA when insulin-depleted equine serum was tested. Caution is recommended when evaluating high insulin concentrations measured with the RIA or ELISAs.
Authors:
Kate E Borer-Weir; Simon R Bailey; Nicola J Menzies-Gow; Patricia A Harris; Jonathan Elliott
Related Documents :
23001546 - Reduced plasma ghrelin levels on day 1 after esophagectomy: a new predictor of prolonge...
23247296 - Ibuprofen reduces zebrafish pge(2) levels but steroid hormone levels and reproductive p...
24376856 - Mmp mediated degradation of type iv collagen alpha 1 and alpha 3 chains reflects baseme...
23267156 - Prognostic impact of circulating mir-21 in the plasma of patients with gastric carcinoma.
6231426 - The effects of depo-provera on serum protein levels in nigerian women.
21442636 - Circulating levels of mcp-1, sil-2r, il-15, and il-8 predict anemia response to pomalid...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of veterinary research     Volume:  73     ISSN:  1943-5681     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1596-602     Citation Subset:  IM    
Affiliation:
Department of Veterinary Clinical Sciences, Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, AL9 7TA, England.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Assessment of blood pool, soft tissue, and skeletal uptake of sodium fluoride F 18 with positron emi...
Next Document:  Association of airborne concentration of virulent Rhodococcus equi with location (stall versus paddo...