Document Detail


Evaluation of a commercial radiometric system for primary isolation of mycobacteria over a fifteen-year period.
MedLine Citation:
PMID:  9105848     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rate of recovery and time to detection of mycobacteria from clinical specimens were analysed for specimens inoculated into both radiometric Middlebrook 7H12 (Bactec 12B, originally 12A) medium and Lowenstein-Jensen egg-based medium (with and without sodium pyruvate) over the 15-year period from 1980 to 1994. A total of 19,679 Bactec vials were inoculated together with Lowenstein-Jensen slopes, with 2198 mycobacterial isolates detected. The mean times to detection for Bactec and Lowenstein-Jensen were 11.7 days and 23.9 days, respectively. In 195 cases mycobacteria were isolated from Bactec and not from Lowenstein-Jensen, whereas the reverse was true in 42 cases. The number of contaminated Bactec vials was 488 and the number of contaminated Lowenstein-Jensen slopes 448.
Authors:
A M Middleton; M V Chadwick; H Gaya
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology     Volume:  16     ISSN:  0934-9723     ISO Abbreviation:  Eur. J. Clin. Microbiol. Infect. Dis.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-06-20     Completed Date:  1997-06-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8804297     Medline TA:  Eur J Clin Microbiol Infect Dis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  166-70     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Royal Brompton Hospital, London, UK.
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques
Culture Media / metabolism
Humans
Laboratory Techniques and Procedures / methods*
Mycobacterium / growth & development*
Mycobacterium Infections / diagnosis*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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