Document Detail

Evaluation of a commercial radiometric system for primary isolation of mycobacteria over a fifteen-year period.
MedLine Citation:
PMID:  9105848     Owner:  NLM     Status:  MEDLINE    
The rate of recovery and time to detection of mycobacteria from clinical specimens were analysed for specimens inoculated into both radiometric Middlebrook 7H12 (Bactec 12B, originally 12A) medium and Lowenstein-Jensen egg-based medium (with and without sodium pyruvate) over the 15-year period from 1980 to 1994. A total of 19,679 Bactec vials were inoculated together with Lowenstein-Jensen slopes, with 2198 mycobacterial isolates detected. The mean times to detection for Bactec and Lowenstein-Jensen were 11.7 days and 23.9 days, respectively. In 195 cases mycobacteria were isolated from Bactec and not from Lowenstein-Jensen, whereas the reverse was true in 42 cases. The number of contaminated Bactec vials was 488 and the number of contaminated Lowenstein-Jensen slopes 448.
A M Middleton; M V Chadwick; H Gaya
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology     Volume:  16     ISSN:  0934-9723     ISO Abbreviation:  Eur. J. Clin. Microbiol. Infect. Dis.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-06-20     Completed Date:  1997-06-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8804297     Medline TA:  Eur J Clin Microbiol Infect Dis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  166-70     Citation Subset:  IM    
Department of Microbiology, Royal Brompton Hospital, London, UK.
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MeSH Terms
Bacteriological Techniques
Culture Media / metabolism
Laboratory Techniques and Procedures / methods*
Mycobacterium / growth & development*
Mycobacterium Infections / diagnosis*
Sensitivity and Specificity
Reg. No./Substance:
0/Culture Media

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