Document Detail


Evaluation of commercial conjugates for fluorescent antibody detection of slamonellae.
MedLine Citation:
PMID:  4133829     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fluorescent antibody (FA) reagents for Salmonella produced by Difco, Sylvana, and Clinical Sciences, Inc., were evaluated for physicochemical and performance characteristics. The Difco panvalent (A through 064) and the Difco polyvalent (A through S) were similar in physicochemical characteristics. They had less than 60% gamma globulin with 3% albumin and had fluorescein to protein (F/P) ratios of less than 10. The Sylvana conjugate had 81% gamma globulin with less than 1% albumin. Its F/P was 33.9. The Clinical Sciences reagent contained 75% unlabeled albumin as packaged in the Fluoro-kit. Analysis of the original conjugate showed 86.5% gamma globulin with only 0.5% albumin. The (F/P) was 32.8. The performance characteristics were determined by using a variety of Enterobacteriaceae and food and feed samples. All conjugates stained the homologous Salmonella strains. The majority of cross-reactions were limited primarily to the Arizona, Citrobacter, and Escherichia coli groups. The Difco panvalent was more reactive with heterologous organisms. It stained 89% of the Arizona compared with 42% stained by the Difco polyvalent (A through S) and 39% stained by the Sylvana and Clinical Sciences reagents. We found 90% agreement between FA and culture when the Difco polyvalent was used to examine food and feed samples and 94% agreement when the Clinical Sciences Fluoro-kit was used on another group of samples.
Authors:
B M Thomason; G A Hebert
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  27     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1974 May 
Date Detail:
Created Date:  1974-07-31     Completed Date:  1974-07-31     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  862-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Antibodies, Bacterial / analysis
Bacterial Proteins / analysis
Enterobacteriaceae / immunology
Escherichia / immunology
Escherichia coli / immunology
Evaluation Studies as Topic
Fluoresceins
Fluorescent Antibody Technique*
Food Microbiology*
Humans
Immunoglobulins*
Indicators and Reagents / analysis*
Proteus / immunology
Pseudomonas / immunology
Salmonella / immunology,  isolation & purification*
Serotyping
Serratia / immunology
Serum Albumin
Shigella / immunology
Species Specificity
gamma-Globulins
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Bacterial Proteins; 0/Fluoresceins; 0/Immunoglobulins; 0/Indicators and Reagents; 0/Serum Albumin; 0/gamma-Globulins
Comments/Corrections

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