Document Detail


Evaluation of a commercial IgG/IgM Western blot assay for early postnatal diagnosis of congenital toxoplasmosis.
MedLine Citation:
PMID:  12649715     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to evaluate a commercial Western blot IgG/IgM assay for use in the early serological diagnosis of congenital toxoplasmosis. This assay compares the immunological profile of mother and infant and allows differentiation between passive transmitted maternal antibodies and newly synthesized antibodies of the infant within the first 3 months of life. Over a 6-year period (1995-2001), the sera from 169 mothers and their 175 offspring (6 had twins) were examined for specific anti- Toxoplasma gondii IgG, IgM and IgA antibodies with an enzyme-linked immunosorbent assay or an immunosorbent agglutination assay. All mothers had primary Toxoplasma infection during pregnancy. Serological and clinical follow-up of the infants during the first year of life confirmed 36 cases of congenital toxoplasmosis. In 139 cases, infection could be ruled out. Three hundred fifty-one paired samples from 175 mother-child pairs were tested retrospectively for IgG and IgM patterns by Toxoplasma Western blot IgG/IgM (LDBIO Diagnostics, France). The results of conventional serological analysis (immunosorbent agglutination assay or enzyme-linked immunosorbent assay) to detect IgM or IgA were compared with the results of the Toxoplasma Western blot IgG/IgM on samples obtained within the first 3 months of life. The performance of the combination of the two methods was also assessed. At birth, the sensitivity values of conventional serological analysis and the Toxoplasma Western blot were 52% and 67%, with specificity values being 99% and 96%, respectively. Combination of the Western blot and conventional serological analysis increased the sensitivity at birth to 78% and within the first 3 months of life to 85%. Overall, the combination of both methods detected 94% of congenital infections. Therefore, this commercial Western blot represents a useful tool for early postnatal diagnosis of congenital toxoplasmosis.
Authors:
V Rilling; K Dietz; D Krczal; F Knotek; G Enders
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2003-03-05
Journal Detail:
Title:  European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology     Volume:  22     ISSN:  0934-9723     ISO Abbreviation:  Eur. J. Clin. Microbiol. Infect. Dis.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-21     Completed Date:  2003-07-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8804297     Medline TA:  Eur J Clin Microbiol Infect Dis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  174-80     Citation Subset:  IM    
Affiliation:
Labor Prof. G. Enders und Partner und Institut für Virologie, Infektiologie und Epidemiologie e.V., Rosenbergstrasse 85, 70193, Stuttgart, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Protozoan / analysis*
Blotting, Western*
Case-Control Studies
Enzyme-Linked Immunosorbent Assay
Female
Fetal Blood / immunology,  parasitology
Humans
Immunoglobulin A / analysis
Immunoglobulin G / analysis*
Immunoglobulin M / analysis*
Infant, Newborn
Postnatal Care
Pregnancy
Sensitivity and Specificity
Toxoplasmosis, Congenital / diagnosis*
Chemical
Reg. No./Substance:
0/Antibodies, Protozoan; 0/Immunoglobulin A; 0/Immunoglobulin G; 0/Immunoglobulin M

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