Document Detail


Evaluation of a commercial Echinococcus Western Blot assay for serological follow-up of patients with alveolar echinococcosis.
MedLine Citation:
PMID:  18799646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A total of 20 patients with alveolar echinococcosis in different clinical stages according to the WHO-PNM staging system (P, parasitic mass in the liver; N, involvement of neighboring organs; M, metastasis) were followed up serologically with the commercial Echinococcus Western Blot IgG assay and a crude antigen extract enzyme-linked immunosorbent assay (ELISA). The cohort included patients after curative resection and patients who had unresectable lesions with stable disease or progressive infection. There were visible correlations of the crude antigen ELISA index and the presence and intensity of diagnostic bands in the Western blot. In most patients after curative resection, bands at 7, 16, and 18 kDa markedly decreased or vanished after 1 to 4 years. In a patient with a nonviable lesion (it died out), bands at 16 and 18 kDa vanished after 4 years. Among individuals with unresectable lesions but stable disease under antiparasitic chemotherapy, a decrease of all diagnostic bands was visible after 2 to 3 years in half of the patients, whereas the other half had unchanged blot results after 4 to 6 years. Patients with progressive disease showed increasing intensities of bands at 16, 18, and 7 kDa. The change of banding patterns was not influenced by the PNM stage in patients after curative surgery or with unresectable lesions. Our data indicate a correlation of the 7-, 16-, and 18-kDa-Western blot bands with disease activity independent of the PNM stage. This study demonstrated the usefulness of the Echinococcus Western Blot IgG assay as an additional serological test for the follow-up of patients with alveolar echinococcosis.
Authors:
Dennis Tappe; Beate Grüner; Peter Kern; Matthias Frosch
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article     Date:  2008-09-17
Journal Detail:
Title:  Clinical and vaccine immunology : CVI     Volume:  15     ISSN:  1556-679X     ISO Abbreviation:  Clin. Vaccine Immunol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-06     Completed Date:  2008-12-01     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  101252125     Medline TA:  Clin Vaccine Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1633-7     Citation Subset:  IM    
Affiliation:
Institute of Hygiene and Microbiology, University of Würzburg, Würzburg, Germany. dtappe@hygiene.uni-wuerzburg.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Animals
Antibodies, Helminth / blood*
Blotting, Western / methods*
Cohort Studies
Echinococcosis, Pulmonary / drug therapy,  immunology*,  surgery
Enzyme-Linked Immunosorbent Assay
Female
Follow-Up Studies
Humans
Immunoglobulin G / blood*
Male
Middle Aged
Chemical
Reg. No./Substance:
0/Antibodies, Helminth; 0/Immunoglobulin G
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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