| Evaluation of chromatin condensation in human spermatozoa: a flow cytometric assay using acridine orange staining. | |
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MedLine Citation:
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PMID: 9239707 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The quality of sperm chromatin is an important factor in fertilization and is especially critical where one spermatozoon is artificially selected for fertilizing an egg (as in intracytoplasmic sperm injection). In this study, flow cytometry after staining of human spermatozoa with Acridine Orange was used to study chromatin structure. A method is described for estimating the percentage of cells in a human sperm sample that have completed epididymal maturation in regard to chromatin condensation. Of the 121 samples of the semen that were examined, nine contained a higher percentage of hypocondensed spermatozoa and six samples contained elevated amounts of hypercondensed spermatozoa. In addition to aberrancies in chromatin condensation other defects showed up as satellite populations of spermatozoa with higher than normal ratios of red/green fluorescence after Acridine Orange staining. Such defects were found in 15 semen samples. The use of swim-up and Percoll gradient centrifugation methods was shown to improve the percentage of spermatozoa with normal chromatin structure in some samples with poor initial quality. |
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Authors:
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R Golan; L Shochat; R Weissenberg; Y Soffer; Z Marcus; Y Oschry; L M Lewin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular human reproduction Volume: 3 ISSN: 1360-9947 ISO Abbreviation: Mol. Hum. Reprod. Publication Date: 1997 Jan |
Date Detail:
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Created Date: 1997-09-02 Completed Date: 1997-09-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9513710 Medline TA: Mol Hum Reprod Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 47-54 Citation Subset: IM |
Affiliation:
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Department of Clinical Biochemistry, Sackler Medical School, Tel Aviv University, Ramat Aviv, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acridine Orange Chromatin / ultrastructure* Flow Cytometry Fluorescent Dyes Humans Male Spermatozoa* / ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Fluorescent Dyes; 65-61-2/Acridine Orange |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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