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Evaluation of choroidal endothelial cell proliferation after exposure to varying doses of proton beam radiation.
MedLine Citation:
PMID:  20829738     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
PURPOSE: Focal epiretinal radiation has emerged as a promising tool in the management of choroidal neovascularization associated with age-related macular degeneration. However, the dosages tested are not backed by cell culture studies used in the clinical setting empirically.
METHODS: Choroidal endothelial cells (RF6A) were maintained in a log scale and exposed to a single fraction of 2, 4, 8, and 12 cobalt gray-equivalent of proton radiation with an internal control. Cell viability was quantified using Vi-cell XR and neutral red assay at days 5, 9, and 12 after radiation. Mitochondrial viability using WST-1 and reactive oxygen species levels using dihydrorhodamine 123 were measured at similar intervals.
RESULTS: By using neutral red assay, on day 12, the percentages of viable cells compared with control were 100.1 ± 5.7%, 96.7 ± 23.3%, 27.6 ± 6.6%, and 19.5 ± 3% at radiation doses of 2, 4, 8, and 12 cobalt gray-equivalent, respectively (P < 0.001). Increase in reactive oxygen species levels correlated with the number of dead cells implicating reactive oxygen species as an intermediary molecule (r = 0.85-0.96).
CONCLUSION: Our study shows sensitivity of cultured choroidal endothelial cells to proton beam radiation at doses of 8 and 12 cobalt gray-equivalent in an in vitro model.
Authors:
Kakarla V Chalam; Sankarathi Balaiya; Robert S Malyappa; Wen Hsi; Vikram S Brar; Ravi K Murthy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Retina (Philadelphia, Pa.)     Volume:  31     ISSN:  1539-2864     ISO Abbreviation:  Retina (Philadelphia, Pa.)     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309919     Medline TA:  Retina     Country:  United States    
Other Details:
Languages:  eng     Pagination:  169-76     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USA. kchalam@jax.ufl.edu
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