| Evaluation of choroidal endothelial cell proliferation after exposure to varying doses of proton beam radiation. | |
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MedLine Citation:
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PMID: 20829738 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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PURPOSE: Focal epiretinal radiation has emerged as a promising tool in the management of choroidal neovascularization associated with age-related macular degeneration. However, the dosages tested are not backed by cell culture studies used in the clinical setting empirically. METHODS: Choroidal endothelial cells (RF6A) were maintained in a log scale and exposed to a single fraction of 2, 4, 8, and 12 cobalt gray-equivalent of proton radiation with an internal control. Cell viability was quantified using Vi-cell XR and neutral red assay at days 5, 9, and 12 after radiation. Mitochondrial viability using WST-1 and reactive oxygen species levels using dihydrorhodamine 123 were measured at similar intervals. RESULTS: By using neutral red assay, on day 12, the percentages of viable cells compared with control were 100.1 ± 5.7%, 96.7 ± 23.3%, 27.6 ± 6.6%, and 19.5 ± 3% at radiation doses of 2, 4, 8, and 12 cobalt gray-equivalent, respectively (P < 0.001). Increase in reactive oxygen species levels correlated with the number of dead cells implicating reactive oxygen species as an intermediary molecule (r = 0.85-0.96). CONCLUSION: Our study shows sensitivity of cultured choroidal endothelial cells to proton beam radiation at doses of 8 and 12 cobalt gray-equivalent in an in vitro model. |
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Authors:
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Kakarla V Chalam; Sankarathi Balaiya; Robert S Malyappa; Wen Hsi; Vikram S Brar; Ravi K Murthy |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Retina (Philadelphia, Pa.) Volume: 31 ISSN: 1539-2864 ISO Abbreviation: Retina (Philadelphia, Pa.) Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309919 Medline TA: Retina Country: United States |
Other Details:
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Languages: eng Pagination: 169-76 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USA. kchalam@jax.ufl.edu |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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