Document Detail

Evaluation of cell recycle on Thermomyces lanuginosus Xylanase A production by Pichia pastoris GS 115.
MedLine Citation:
PMID:  16915642     Owner:  NLM     Status:  MEDLINE    
This work aims to evaluate cell recycle of a recombinant strain of Pichia pastoris GS115 on the Xylanase A (XynA) production of Thermomyces lanuginosus IOC-4145 in submerged fermentation. Fed-batch processes were carried out with methanol feeding at each 12 h and recycling cell at 24, 48, and 72 h. Additionally, the influence of the initial cell concentration was investigated. XynA production was not decreased with the recycling time, during four cell recycles, using an initial cell concentration of 2.5 g/L. The maximum activity was 14,050 U/L obtained in 24 h of expression. However, when the initial cell concentration of 0.25 g/L was investigated, the enzymatic activity was reduced by 30 and 75% after the third and fourth cycles, respectively. Finally, it could be concluded that the initial cell concentration influenced the process performance and the interval of cell recycle affected enzymatic production.
Verônica Ferreira; Patricia C Nolasco; Aline M Castro; Juliana N C Silva; Alexandre S Santos; Mônica C T Damaso; Nei Pereira Júnior
Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  129-132     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-08-18     Completed Date:  2006-09-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  226-33     Citation Subset:  IM    
Escola de Química-Universidade Federal do Rio de Janeiro, Caixa Postal 68542, CEP 21945-970, Rio de Janeiro, RJ, Brazil.
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MeSH Terms
Ascomycota / enzymology*,  genetics
Bioreactors / microbiology*
Cell Culture Techniques / methods*
Cell Proliferation
Cell Survival
Endo-1,4-beta Xylanases / biosynthesis*,  genetics
Pichia / physiology*
Protein Engineering / methods*
Reg. No./Substance:
EC,4-beta Xylanases

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