Document Detail

Evaluation of cell proliferation in breast carcinoma. Comparison of Ki-67 immunohistochemical study, DNA flow cytometric analysis, and mitotic count.
MedLine Citation:
PMID:  2406010     Owner:  NLM     Status:  MEDLINE    
Growth kinetics of 102 breast carcinomas were studied by immunohistochemical analysis with the monoclonal antibody Ki-67, which reacts with a nuclear antigen in proliferating cells. The results were correlated with ploidy and S-phase fraction (SPF) analyzed by DNA flow cytometric study and with mitotic count analyzed by light microscopic study. The proportion of Ki-67-positive cells (Ki-67 score) in breast carcinomas varied from 0.6% to 80% (median, 6.3%). Ki-67 scores were significantly higher in the DNA aneuploid than in the DNA diploid tumors. Ki-67 scores correlated significantly with tumor SPF in DNA aneuploid tumors. In DNA diploid tumors SPF showed no correlation with Ki-67 score. High Ki-67 scores were associated with high mitotic counts (P less than 0.0001) and histologic grade (P less than 0.0001). Nuclear pleomorphism, tubule formation, or lymph node status were not correlated with Ki-67 score. In conclusion, Ki-67 immunostaining correlates with other measures of cell proliferation (SPF, mitotic count) supporting its clinical significance.
J J Isola; H J Helin; M J Helle; O P Kallioniemi
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cancer     Volume:  65     ISSN:  0008-543X     ISO Abbreviation:  Cancer     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-03-27     Completed Date:  1990-03-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374236     Medline TA:  Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1180-4     Citation Subset:  AIM; IM    
Department of Biomedical Sciences, University of Tampere, Finland.
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MeSH Terms
Aged, 80 and over
Antibodies, Monoclonal / diagnostic use
Breast Neoplasms / genetics,  pathology*
Carcinoma / genetics,  pathology*
DNA, Neoplasm / analysis
Flow Cytometry
Immunoenzyme Techniques
Lymphatic Metastasis
Middle Aged
Mitotic Index
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/DNA, Neoplasm

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