| Evaluation of cell-free protein synthesis using PDMS-based microreactor arrays. | |
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MedLine Citation:
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PMID: 18270416 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A living cell has numerous proteins, only a few thousand of which have been identified to date. Cell-free protein synthesis is a useful and promising technique to discover and produce various proteins that might be beneficial for biotechnological, pharmaceutical, and medical applications. For this study, we evaluated the performance and the general applicability of our previously developed microreactor array chip to cell-free protein synthesis by comparisons with a commercially available system. The microreactor array chip comprises a temperature control chip made of glass and a disposable reaction chamber chip made of polydimethylsiloxane (PDMS). For evaluation of the microreactor array chip, rat adipose-type fatty acid binding protein, glyceraldehyde-3-phosphate dehydrogenase, cyclophilin, and firefly luciferase were synthesized from their respective DNA templates using a cell-free extract prepared from Escherichia coli. All these proteins were synthesized in the microreactor array chip, and their respective amounts and yields were investigated quantitatively. |
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Authors:
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Takatoki Yamamoto; Mami Hino; Rei Kakuhata; Takahiko Nojima; Yasuo Shinohara; Yoshinobu Baba; Teruo Fujii |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry Volume: 24 ISSN: 0910-6340 ISO Abbreviation: Anal Sci Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-02-13 Completed Date: 2008-05-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8511078 Medline TA: Anal Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 243-6 Citation Subset: IM |
Affiliation:
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Center for International Research on MicroMechatronics, Institute of Industrial Science, The University of Tokyo, Meguro, Tokyo, Japan. takatoki@iis.u-tokyo.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell-Free System / chemistry Cyclophilins / chemical synthesis*, chemistry Dimethylpolysiloxanes / chemistry* Fatty Acid-Binding Proteins / chemical synthesis*, chemistry Glycerol-3-Phosphate Dehydrogenase (NAD+) / chemical synthesis*, chemistry Luciferases / chemical synthesis*, chemistry Microfluidic Analytical Techniques / instrumentation*, methods Rats Reproducibility of Results Sensitivity and Specificity Silicones / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Dimethylpolysiloxanes; 0/Fatty Acid-Binding Proteins; 0/Silicones; 63148-62-9/baysilon; EC 1.1.1.8/Glycerol-3-Phosphate Dehydrogenase (NAD+); EC 1.13.12.-/Luciferases; EC 5.2.1.-/Cyclophilins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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