| Evaluation of the causal relationship between crocidolite asbestos-induced lipid peroxidation and toxicity to macrophages. | |
| | |
MedLine Citation:
|
PMID: 2540689 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In vitro, crocidolite asbestos toxicity to macrophages is mediated by the production of reactive oxygen metabolites. We examined whether exposure of macrophages to crocidolite asbestos induced lipid peroxidation as measured by the thiobarbituric acid assay. When elicited mouse peritoneal macrophages were exposed to crocidolite, a dose- and time-dependent increase in lipid peroxidation breakdown products accompanied cell death. Superoxide dismutase plus catalase or deferoxamine prevented both lipid peroxidation and loss of viability caused by crocidolite. We tested whether crocidolite-induced lipid peroxidation was causally responsible for cell death. Macrophages were not killed by crocidolite when incubated with 10 mM 3-aminobenzamide. The level of thiobarbituric acid-reactive material was the same, however, for cells incubated with crocidolite in the presence or absence of 3-aminobenzamide. When macrophagaes were pretreated for 24 h with 25 microM vitamin E and then incubated with crocidolite, no thiobarbituric acid-reactive products were detected. Vitamin E, however, did not prevent crocidolite cytotoxicity. These results suggest that exposure of macrophages to crocidolite asbestos produces lipid peroxidation as measured by thiobarbituric acid-reactive products. This reaction, however, is not directly responsible for irreversible injury in this model system. |
| | |
Authors:
|
L A Goodglick; L A Pietras; A B Kane |
Related Documents
:
|
21158399 - Aggregation and ph responsive disassembly of a new acid-labile surfactant synthesized b... 21331199 - Ultraviolet spectrophotometric method for determination of gelatin crosslinking in the ... 21218219 - Fabrication of a palladium nanoparticle/graphene nanosheet hybrid via sacrifice of a co... 21426189 - Titanium-catalyzed esterification and transesterification reactions facilitated using m... 21485279 - Identification and quantitative determination of lignans in cedrus atlantica resins usi... 15070069 - Glucose-stimulated acrolein production from unsaturated fatty acids. 6209989 - Csf neurochemistry in depressed, manic, and schizophrenic patients compared with that o... 19711199 - Fermentation of sugarcane bagasse and chicken manure to calcium carboxylates under ther... 11308029 - Elucidation of the role of functional amino acid residues of the small sialidase from c... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The American review of respiratory disease Volume: 139 ISSN: 0003-0805 ISO Abbreviation: Am. Rev. Respir. Dis. Publication Date: 1989 May |
Date Detail:
|
Created Date: 1989-06-07 Completed Date: 1989-06-07 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0370523 Medline TA: Am Rev Respir Dis Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1265-73 Citation Subset: AIM; IM |
Affiliation:
|
Department of Pathology, Brown University, Providence, Rhode Island 02912. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Asbestos / toxicity* Asbestos, Crocidolite Cell Survival / drug effects Cells, Cultured Dose-Response Relationship, Drug Drug Interactions Lipid Peroxidation / drug effects* Lipids / analysis Macrophages / analysis, drug effects*, metabolism Mice Time Factors |
| Grant Support | |
ID/Acronym/Agency:
|
R01 ES-03189/ES/NIEHS NIH HHS; T32 GM-07601/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Lipids; 12001-28-4/Asbestos, Crocidolite; 1332-21-4/Asbestos |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Asbestos fibers and silica particles stimulate rat alveolar macrophages to release tumor necrosis fa...
Next Document: Surgical treatment of 48 primary peripheral, non-small cell lung cancers equal to or greater than 8 ...