Document Detail

Evaluation of the causal relationship between crocidolite asbestos-induced lipid peroxidation and toxicity to macrophages.
MedLine Citation:
PMID:  2540689     Owner:  NLM     Status:  MEDLINE    
In vitro, crocidolite asbestos toxicity to macrophages is mediated by the production of reactive oxygen metabolites. We examined whether exposure of macrophages to crocidolite asbestos induced lipid peroxidation as measured by the thiobarbituric acid assay. When elicited mouse peritoneal macrophages were exposed to crocidolite, a dose- and time-dependent increase in lipid peroxidation breakdown products accompanied cell death. Superoxide dismutase plus catalase or deferoxamine prevented both lipid peroxidation and loss of viability caused by crocidolite. We tested whether crocidolite-induced lipid peroxidation was causally responsible for cell death. Macrophages were not killed by crocidolite when incubated with 10 mM 3-aminobenzamide. The level of thiobarbituric acid-reactive material was the same, however, for cells incubated with crocidolite in the presence or absence of 3-aminobenzamide. When macrophagaes were pretreated for 24 h with 25 microM vitamin E and then incubated with crocidolite, no thiobarbituric acid-reactive products were detected. Vitamin E, however, did not prevent crocidolite cytotoxicity. These results suggest that exposure of macrophages to crocidolite asbestos produces lipid peroxidation as measured by thiobarbituric acid-reactive products. This reaction, however, is not directly responsible for irreversible injury in this model system.
L A Goodglick; L A Pietras; A B Kane
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American review of respiratory disease     Volume:  139     ISSN:  0003-0805     ISO Abbreviation:  Am. Rev. Respir. Dis.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-06-07     Completed Date:  1989-06-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370523     Medline TA:  Am Rev Respir Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1265-73     Citation Subset:  AIM; IM    
Department of Pathology, Brown University, Providence, Rhode Island 02912.
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MeSH Terms
Asbestos / toxicity*
Asbestos, Crocidolite
Cell Survival / drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Drug Interactions
Lipid Peroxidation / drug effects*
Lipids / analysis
Macrophages / analysis,  drug effects*,  metabolism
Time Factors
Grant Support
Reg. No./Substance:
0/Lipids; 12001-28-4/Asbestos, Crocidolite; 1332-21-4/Asbestos

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