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Evaluation of canine antimicrobial peptides in infected and noninfected chronic atopic skin.
MedLine Citation:
PMID:  23331678     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background -  Antimicrobial peptides (AMPs) are small immunomodulatory peptides produced by epithelial and immune cells. β-Defensins (BDs) and cathelicidins (Caths) are the most studied AMPs. Recently, increased cutaneous expression of AMPs was reported in atopic humans and in beagles with experimentally induced atopy. Hypothesis/Objectives -  Our goal was to analyse mRNA expression and protein levels of canine (c)BD1-like, cBD2-like/122, cBD3-like, cBD103 and cCath in healthy and naturally affected atopic dogs, with and without active skin infection, along with their distribution in the epidermis using indirect immunofluorescence. Animals -  Skin biopsies were taken from 14 healthy and 11 atopic privately owned dogs. Methods -  The mRNA levels of cBD1-like, cBD2-like/122, cBD3-like, cBD103 and cCath were quantified using quantitative real-time PCR. The protein levels of cBD3-like and cCath were analysed by relative competitive inhibition enzyme-linked immunosorbent assay, while the distributions of cBD2-like/122, cBD3-like and cCath were detected by indirect immunofluorescence. Results -  Dogs with atopic dermatitis had significantly greater mRNA expression of cBD103 (P = 0.04) than control dogs. Furthermore, atopic skin with active infection had a higher cBD103 mRNA expression (P = 0.01) and a lower cBD1-like mRNA expression (P = 0.04) than atopic skin without infection. No significant differences in protein levels (cBD3-like and cCath) or epidermal distribution of AMPs (cBD2-like/122, cBD3-like and cCath) were seen between healthy and atopic dogs. Conclusions and clinical importance -  Expression of cBD103 mRNA was greater, while expression of cBD1-like mRNA was lower in dogs with atopic dermatitis that had active infections. Work is needed to clarify the biological mechanisms and possible therapeutic options to maintain a healthy canine skin.
Authors:
Domenico Santoro; David Bunick; Thomas K Graves; Mariangela Segre
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Veterinary dermatology     Volume:  24     ISSN:  1365-3164     ISO Abbreviation:  Vet. Dermatol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426187     Medline TA:  Vet Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  39-e10     Citation Subset:  IM    
Copyright Information:
© 2013 The Authors. Veterinary Dermatology © 2013 ESVD and ACVD.
Affiliation:
Departments of Veterinary Clinical Medicine Comparative Biosciences Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
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