Document Detail


Evaluation of bone regeneration using the rat critical size calvarial defect.
MedLine Citation:
PMID:  23018195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Animal models that are reliably reproducible, appropriate analogs to the clinical condition they are used to investigate, and that offer minimal morbidity and periprocedural mortality to the subject, are the keystone to the preclinical development of translational technologies. For bone tissue engineering, a number of small animal models exist. Here we describe the protocol for one such model, the rat calvarial defect. This versatile model allows for evaluation of biomaterials and bone tissue engineering approaches within a reproducible, non-load-bearing orthotopic site. Crucial steps for ensuring appropriate experimental control and troubleshooting tips learned through extensive experience with this model are provided. The surgical procedure itself takes ∼30 min to complete, with ∼2 h of perioperative care, and tissue collection is generally performed 4-12 weeks postoperatively. Several analytical techniques are presented, which evaluate the cellular and extracellular matrix components, functionality and mineralization, including histological, mechanical and radiographic methods.
Authors:
Patrick P Spicer; James D Kretlow; Simon Young; John A Jansen; F Kurtis Kasper; Antonios G Mikos
Related Documents :
18438215 - Prediction of anaerobic power values from an abbreviated want protocol.
21868935 - A new heuristic for inferring regular grammars.
23666785 - Brede tools and federating online neuroinformatics databases.
24325985 - Automated analysis of intracellular calcium fluorescence in rat organotypic hippocampal...
19011925 - Evaluating automated parameter constraining procedures of neuron models by experimental...
18595055 - Parametric analysis of the errors associated with the michaelis-menten equation.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-09-27
Journal Detail:
Title:  Nature protocols     Volume:  7     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  2013-01-28     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1918-29     Citation Subset:  IM    
Affiliation:
Department of Bioengineering, Rice University, Houston, Texas, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bone Regeneration*
Models, Animal*
Rats*
Rats, Inbred F344
Reproducibility of Results
Skull / physiology*,  surgery
Tissue Engineering / methods*
Grant Support
ID/Acronym/Agency:
R01 AR042639/AR/NIAMS NIH HHS; R01 AR42639/AR/NIAMS NIH HHS; R01 DE015164/DE/NIDCR NIH HHS; R01 DE015164/DE/NIDCR NIH HHS; R01 DE017441/DE/NIDCR NIH HHS; R01 DE017441/DE/NIDCR NIH HHS; R21 AR056076/AR/NIAMS NIH HHS; R21 AR56076/AR/NIAMS NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The GLIB technique for genome-wide mapping of 5-hydroxymethylcytosine.
Next Document:  Observation of phonon sideband emission in intrinsic InN nanowires: a photoluminescence and micro-Ra...