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Evaluation of the blood volume effect on the diagnosis of bacteremia in automated blood culture systems.
MedLine Citation:
PMID:  22738875     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Blood culture volume is the most important variable in detecting bacteremia and fungemia. However, the majority of hospitals in Taiwan do not meet the criteria for an ideal blood culture volume (8-10 mL per bottle, two bottles per set) during collection. METHODS: The object of this study is to initiate an educational program for healthcare workers to increase blood volume collection and to evaluate the relationship between blood volumes and bacteremia recovery rate for detecting bacteremia and fungemia effectively by using the BD BACTEC 9240 blood culture system. RESULTS: After education, the blood sample volume ≥5 mL group increased from 2.93% to 71.24%. For a total of 4,844 bottles, the relative improvement in recovery rate for detection has increased by 17.81% between the <5 mL group and the ≥5 mL group. The recovery rates for the low-volume (<3 mL), mid-volume (3-7 mL), high-volume (8-10 mL) and extreme high-volume (>10 mL) groups are 13.31%, 15.02%, 17.68%, and 14.96%, respectively. CONCLUSION: With good blood collection practice, our study found that blood volume obtained was in direct proportion to recovery rate for the detection of bacteremia and fungemia.
Authors:
Hsiu-Hsien Lin; Yen-Fang Liu; Ni Tien; Cheng-Mao Ho; Ling-Nu Hsu; Jang-Jih Lu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-25
Journal Detail:
Title:  Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi     Volume:  -     ISSN:  1995-9133     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100956211     Medline TA:  J Microbiol Immunol Infect     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
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