Document Detail


Evaluation of the biological behavior of decellularized pulmonary homografts: an experimental sheep model.
MedLine Citation:
PMID:  21103747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: The cryopreserved homograft is a good valve substitute due attributes like excellent hemodynamics, low incidence of thromboembolic events, infection resistance and good mid-term durability. However, progressive homograft degeneration and fibrocalcification may occur, particularly in the childhood and young adults. Their antigenicity triggers an immunological reaction that plays an important role in their degeneration and failure. The decellularization process was proposed to decrease this antigenicity. By the action of detergents and enzymes, this process removes all cellular components from the homograft matrix, diminishing immunogenicity and probably delaying its degeneration.
OBJECTIVE: The objective of this experimental and descriptive study is to evaluate the biological and functional behavior of decellularized pulmonary homografts (Decell-H), treated by a sodium dodecil sulfate solution (0.1%), developed in our University (Pontifícia Universidade Católica do Paraná). For the characterization of Decell-H performance, parameters like recellularization, calcification, and echocardiographic data will be analyzed.
METHODS: Eight juvenile sheep were submitted to the implantation of the Decell-H sutured into orthotopic position, through a left thoracotomy and with cardiopulmonary bypass support. They were followed-up clinically and by periodical echocardiograms until the explantation, which were performed in different time for every two sheep: seven, 30, 90 and 180 postoperative days. For histological analysis we used Hematoxilin-eosin, Movat and Alizarin-Red staining.
RESULTS: The sheep reached their follow-up period in a good clinical state. There was no valve regurgitation or stenonis by the echocardiogram. The animals submitted to the explantation in 90 and 180 days had a significant somatic growth and these Decell-H(s) had a diameter increase, without central valve insufficiency. Histologically, all homografts preserved their extra-cellular matrix organization and were progressively recellularized, without calcification.
CONCLUSION: In this experimental model, the Decell-H behaved as an excellent valve substitute.
Authors:
Fábio Binhara Navarro; Francisco Diniz Affonso da Costa; Leonardo Andrade Mulinari; Gustavo Klug Pimentel; João Gabriel Roderjan; Eduardo Discher Vieira; Lúcia de Noronha; Nelson Itiro Miyague
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Revista brasileira de cirurgia cardiovascular : órgão oficial da Sociedade Brasileira de Cirurgia Cardiovascular     Volume:  25     ISSN:  1678-9741     ISO Abbreviation:  Rev Bras Cir Cardiovasc     Publication Date:    2010 Jul-Sep
Date Detail:
Created Date:  2010-11-24     Completed Date:  2011-04-06     Revised Date:  2012-04-02    
Medline Journal Info:
Nlm Unique ID:  9104279     Medline TA:  Rev Bras Cir Cardiovasc     Country:  Brazil    
Other Details:
Languages:  eng; por     Pagination:  377-87     Citation Subset:  IM    
Affiliation:
SBCCV, Hospital Pequeno Príncipe of Curitiba, PR, Brazil. fabiobnavarro@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Echocardiography
Female
Male
Models, Animal
Pulmonary Valve / drug effects,  pathology,  transplantation*
Sheep
Sodium Dodecyl Sulfate / pharmacology*
Surface-Active Agents / pharmacology*
Tissue Engineering / methods*
Transplantation, Homologous
Chemical
Reg. No./Substance:
0/Surface-Active Agents; 151-21-3/Sodium Dodecyl Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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