Document Detail


Evaluation of biohazards in dehydrated biofilms on foodstuff packaging.
MedLine Citation:
PMID:  10791750     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plastic materials used for food packaging are clean but not sterile when the food is just packaged. Accidental wet contamination may occur at every moment between packaging and opening by the consumer: on polyethylene (PET), bacteria may adhere strongly and constitute a biofilm in less than 24 h. By rolling on themselves, PET sheets may contaminate food. We tried to show that contact with salted foodstuffs favoured microbial recovery. Four strains were chosen to perform biofilms on PET: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli. Biofilms were dried up 24 h. Biofilm bacteria were stressed by adhesion, by starvation and by dehydration. However, they were capable of recovery in salted solutions or media, probably because one (or more) stress protected them against another stress. Stress was demonstrated by stress protein production, by mean of electrophoresis, and membrane lesions by mean of flow cytometry. Stress recovery was performed in aqueous salted solutions or salted brain-heart infusion with NaCl 9, 15, 20 and 30 g/l. Staphylococci were more sensitive to these stresses and recovery was a function of salt concentration. Gram-negative bacteria were little affected by stresses; salt effects were less important. If all these biofilms were capable of recovery from stresses in salted media, flexible PET could possibly lead to a health hazard when it is used for wet salt meats, e.g.
Authors:
E Le Magrex-Debar; J Lemoine; M P Gellé; L F Jacquelin; C Choisy
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of food microbiology     Volume:  55     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-05-18     Completed Date:  2000-05-18     Revised Date:  2001-11-14    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  239-43     Citation Subset:  IM; S    
Affiliation:
Laboratoire d'Adhésion Bactérienne, Biomatériaux, IFR 53, Biomolécules, Reims, France.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion
Biofilms*
Food Packaging*
Polyethylene
Chemical
Reg. No./Substance:
9002-88-4/Polyethylene

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