| Evaluation of biohazards in dehydrated biofilms on foodstuff packaging. | |
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MedLine Citation:
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PMID: 10791750 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plastic materials used for food packaging are clean but not sterile when the food is just packaged. Accidental wet contamination may occur at every moment between packaging and opening by the consumer: on polyethylene (PET), bacteria may adhere strongly and constitute a biofilm in less than 24 h. By rolling on themselves, PET sheets may contaminate food. We tried to show that contact with salted foodstuffs favoured microbial recovery. Four strains were chosen to perform biofilms on PET: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli. Biofilms were dried up 24 h. Biofilm bacteria were stressed by adhesion, by starvation and by dehydration. However, they were capable of recovery in salted solutions or media, probably because one (or more) stress protected them against another stress. Stress was demonstrated by stress protein production, by mean of electrophoresis, and membrane lesions by mean of flow cytometry. Stress recovery was performed in aqueous salted solutions or salted brain-heart infusion with NaCl 9, 15, 20 and 30 g/l. Staphylococci were more sensitive to these stresses and recovery was a function of salt concentration. Gram-negative bacteria were little affected by stresses; salt effects were less important. If all these biofilms were capable of recovery from stresses in salted media, flexible PET could possibly lead to a health hazard when it is used for wet salt meats, e.g. |
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Authors:
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E Le Magrex-Debar; J Lemoine; M P Gellé; L F Jacquelin; C Choisy |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of food microbiology Volume: 55 ISSN: 0168-1605 ISO Abbreviation: Int. J. Food Microbiol. Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-05-18 Completed Date: 2000-05-18 Revised Date: 2001-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8412849 Medline TA: Int J Food Microbiol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 239-43 Citation Subset: IM; S |
Affiliation:
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Laboratoire d'Adhésion Bactérienne, Biomatériaux, IFR 53, Biomolécules, Reims, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Adhesion Biofilms* Food Packaging* Polyethylene |
| Chemical | |
Reg. No./Substance:
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9002-88-4/Polyethylene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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