Document Detail

Evaluation of biocompatibility of polypyrrole in vitro and in vivo.
MedLine Citation:
PMID:  14762920     Owner:  NLM     Status:  MEDLINE    
In this study, the biocompatibility of the electrically conductive polymer polypyrrole (PPy) with nerve tissue was evaluated in vitro and in vivo. The extraction solution of PPy powder, which was synthesized chemically, was tested for acute toxicity, subacute toxicity, pyretogen, quantitative measure of cell viability, hemolysis, allergen, and micronuclei. The PPy membrane was synthesized electrochemically on the indium tin oxide conductive borosilicate glass. The dorsal root ganglia from 1-3-day-old Sprague-Dawley rats were cultured above PPy membrane and observed by light or scanning electron microscopy. The PPy-silicone tube (PPy membrane on the inner surface of the silicone tube) also synthesized electrochemically was used to bridge across 10-mm sciatic nerve gap in rats. Twenty-four weeks after the operation to rats, the regenerated tissues were observed by electrophysiological and histological techniques. PPy extraction solution showed no evidence of acute and subacute toxicity, pyretogen, hemolysis, allergen, and mutagenesis, and the Schwann cells from the PPy extraction solution group showed better survival rate and proliferation rate as compared with the saline solution control group. The migration of the Schwann cells and the neurite extension from dorsal root ganglia on the surface of PPy membrane-coated glass was better than those of bare glass. There was only lightly inflammation during 6 months of the postoperation, when the PPy-silicone tube bridged across the gap of the transected sciatic nerve. The regeneration of nerve tissue in the PPy-silicone tube was slightly better than that in the plain silicone tube by means of electrophysiological and histological examination. The results of this study indicate that PPy has a good biocompatibility with rat peripheral nerve tissue and that PPy might be a candidate material for bridging the peripheral nerve gap.
Xioadong Wang; Xioasong Gu; Chunwai Yuan; Shujian Chen; Peiyun Zhang; Tianyi Zhang; Jian Yao; Fen Chen; Gang Chen
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  68     ISSN:  1549-3296     ISO Abbreviation:  -     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-05     Completed Date:  2004-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  411-22     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley Periodicals, Inc.
Jiangsu Key Laboratory of Neuron Regeneration, Nantong Medical College, Nantong 226001, People's Republic of China.
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MeSH Terms
Biocompatible Materials / pharmacology*,  standards
Cell Division / drug effects
Cell Movement / drug effects
Cell Survival / drug effects
Ganglia, Spinal / drug effects*
Materials Testing
Nerve Regeneration / drug effects*
Neurites / drug effects
Peripheral Nervous System
Polymers / pharmacology*,  standards
Pyrroles / pharmacology*,  standards
Rats, Sprague-Dawley
Schwann Cells / cytology,  drug effects
Reg. No./Substance:
0/Biocompatible Materials; 0/Polymers; 0/Pyrroles; 30604-81-0/polypyrrole

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