Document Detail


Evaluation of the bacterial diversity in cecal contents of laying hens fed various molting diets by using bacterial tag-encoded FLX amplicon pyrosequencing.
MedLine Citation:
PMID:  19151343     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Laying hens are typically induced to molt to begin a new egg-laying cycle by withdrawing feed for up to 12 to 14 d. Fasted hens are more susceptible to colonization and tissue invasion by Salmonella enterica serovar Enteritidis. Much of this increased incidence in fasted hens is thought to be due to changes in the native intestinal microflora. An alternative to feed withdrawal involves feeding alfalfa meal crumble to hens, which is indigestible by poultry but provides fermentable substrate to the intestinal microbial population and reduces Salmonella colonization of hens compared with feed withdrawal. The present study was designed to quantify differences in the cecal microbial population of hens (n=12) fed a typical layer ration, undergoing feed withdrawal, or being fed alfalfa crumble by using a novel tag bacterial diversity amplification method. Bacteroides, Prevotella, and Clostridium were the most common genera isolated from all treatment groups. Only the ceca of hens undergoing feed withdrawal (n=4) contained Salmonella. The number of genera present was greatest in the alfalfa crumble-fed group and least in the feed withdrawal group (78 vs. 54 genera, respectively). Overall, the microbial diversity was least and Lactobacillius populations were not found in the hens undergoing feed withdrawal, which could explain much of these hens' sensitivity to colonization by Salmonella.
Authors:
T R Callaway; S E Dowd; R D Wolcott; Y Sun; J L McReynolds; T S Edrington; J A Byrd; R C Anderson; N Krueger; D J Nisbet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Poultry science     Volume:  88     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-19     Completed Date:  2009-03-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  298-302     Citation Subset:  IM    
Affiliation:
Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, Agricultural Research Service, USDA, College Station, TX 77845, USA. Todd.Callaway@ars.usda.gov
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria / classification,  isolation & purification*
Bacterial Typing Techniques / methods,  veterinary*
Biodiversity
Cecum / microbiology*
Chickens / microbiology*
Diet / veterinary*
Female
Nucleic Acid Amplification Techniques
Sequence Analysis, DNA / veterinary*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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