| Evaluation of bacteria-induced enamel demineralization using optical profilometry. | |
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MedLine Citation:
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PMID: 19732947 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Streptococcus mutans is considered a major causative of tooth decay due to its ability to rapidly metabolize carbohydrates such as sucrose. One prominent excreted end product of sucrose metabolism is lactic acid. Lactic acid causes a decrease in the pH of the oral environment with subsequent demineralization of the tooth enamel. Biologically relevant bacteria-induced enamel demineralization was studied. METHODS: Optical profiling was used to measure tooth enamel decay with vertical resolution under one nanometer and lateral features with optical resolution as a result of S. mutans biofilm exposure. Comparison measurements were made using AFM. RESULTS: After 72h of biofilm exposure the enamel displayed an 8-fold increase in the observed roughness average (R(a)), as calculated over the entire measured array. Similarly, the average root mean square (RMS) roughness, R(RMS), of the enamel before and after biofilm exposure for 3 days displayed a 7-fold increase. Further, the direct effect of chemically induced enamel demineralization using biologically relevant organic acids was shown. Optical profiles of the enamel surface after addition of a 30% lactic acid solution showed a significant alteration in the surface topography with a corresponding increase in respective surface roughness statistics. Similar measurements with 10% citric acid over seconds and minutes give insight into the demineralization process by providing quantitative measures for erosion rates: comparing surface height and roughness as metrics. SIGNIFICANCE: The strengths of optical profilometry as an analytical tool for understanding and analyzing biologically relevant processes such as biofilm induced tooth enamel demineralization were demonstrated. |
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Authors:
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Sarah E Cross; Jens Kreth; R Paul Wali; Richard Sullivan; Wenyuan Shi; James K Gimzewski |
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Publication Detail:
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Type: Comparative Study; Journal Article Date: 2009-09-03 |
Journal Detail:
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Title: Dental materials : official publication of the Academy of Dental Materials Volume: 25 ISSN: 1879-0097 ISO Abbreviation: Dent Mater Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-10-28 Completed Date: 2010-01-26 Revised Date: 2011-09-22 |
Medline Journal Info:
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Nlm Unique ID: 8508040 Medline TA: Dent Mater Country: England |
Other Details:
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Languages: eng Pagination: 1517-26 Citation Subset: D |
Affiliation:
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UCLA Department of Chemistry and Biochemistry, Los Angeles, CA 90095, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Animals Biofilms Cattle Citric Acid / adverse effects Dental Enamel / drug effects, microbiology*, ultrastructure Hydrogen-Ion Concentration Imaging, Three-Dimensional Lactic Acid / adverse effects Microscopy, Atomic Force Nanotechnology Optical Phenomena Streptococcus mutans / metabolism, physiology* Sucrose / metabolism Time Factors Tooth Demineralization / microbiology*, pathology Tooth Erosion / microbiology, pathology |
| Grant Support | |
ID/Acronym/Agency:
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R01 DE020102-01/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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50-21-5/Lactic Acid; 57-50-1/Sucrose; 77-92-9/Citric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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