| Evaluation of applied biosystems MicroSeq real-time PCR system for detection of Listeria monocytogenes in food. Performance Tested Method 011002. | |
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MedLine Citation:
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PMID: 22165012 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Increasingly, more food companies are relying on molecular methods, such as PCR, for pathogen detection due to their improved simplicity, sensitivity, and rapid time to results. This report describes the validation of a new Real-Time PCR method to detect Listeria monocytogenes in nine different food matrixes. The complete system consists of the MicroSEQ L. monocytogenes Detection Kit, sample preparation, the Applied Biosystems 7500 Fast Real-Time PCR instrument, and RapidFinder Express software. Two sample preparation methods were validated: the PrepSEQ Nucleic Acid extraction kit and the PrepSEQ Rapid Spin sample preparation kit. The test method was compared to the ISO 11290-1 reference method using an unpaired-study design to detect L. monocytogenes in roast beef, cured bacon, lox (smoked salmon), lettuce, whole cow's milk, dry infant formula, ice cream, salad dressing, and mayonnaise. The MicroSEQ L. monocytogenes Detection Kit and the ISO 11290-1 reference method showed equivalent detection based on Chi-square analysis for all food matrixes when the samples were prepared using either of the two sample preparation methods. An independent validation confirmed these findings on smoked salmon and whole cow's milk. The MicroSEQ kit detected all 50 L. monocytogenes strains tested, and none of the 30 nontargeted bacteria strains. |
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Authors:
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Robert S Tebbs; Priya Balachandran; Lily Y Wong; Patrick Zoder; Manohar R Furtado; Olga V Petrauskene; Yanxiang Cao |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of AOAC International Volume: 94 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2011 Sep-Oct |
Date Detail:
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Created Date: 2011-12-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 1481-9 Citation Subset: IM |
Affiliation:
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Life Technologies Corp., 850 Lincoln Centre Dr, Foster City, CA 94404, USA. Robert.Tebbs@lifetech.com |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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