Document Detail


Evaluation and application of reverse transcription loop-mediated isothermal amplification for detection of noroviruses.
MedLine Citation:
PMID:  17245722     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of norovirus (NV) was developed. In order to design primer sets for the detection of a wide range of NVs, NVs were categorized into three groups, that is, genogroup I (GI), prevalent GII, and minor GII; three sets of primers were developed for each group. Clinical specimens of patients suffering from enteric RNA viruses, such as NV, group A and C rotavirus, and sapovirus were examined using these primer sets. Various genotypes of NVs were detected in clinical specimens from patients infected with NV where no false positive reaction was observed with other enteric RNA viruses. Additionally, 88 samples of acute gastroenteritis outbreaks were analyzed by an RT-LAMP assay and compared with the results of routine RT-PCR. The results of the RT-LAMP assay corresponded well to that of RT-PCR. These findings suggest the practical application of the RT-LAMP assay for the detection of NVs in clinical specimens. Consequently, the RT-LAMP system and conventional detection kits (NVGI and NVGII detection kits; Eiken Chemical Co., Ltd., Japan) were compared. The detection rate of the prevalent and minor GII primer sets was similar to that of the conventional NVGII kit, while the detection rate of the GI primer set is different because it can detect several genotypes better than the conventional NVGI kit. This is an initial report that the RT-LAMP system is able to detect NVs in clinical specimens within a wide range.
Authors:
Tomoko Yoda; Yasuhiko Suzuki; Kenji Yamazaki; Naomi Sakon; Masashi Kanki; Ikuko Aoyama; Teizo Tsukamoto
Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical virology     Volume:  79     ISSN:  0146-6615     ISO Abbreviation:  J. Med. Virol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-01-29     Completed Date:  2007-07-20     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  326-34     Citation Subset:  IM    
Affiliation:
Department of Infectious Diseases, Osaka Prefectural Institute of Public Health, Osaka, Japan. yoda@iph.pref.osaka.jp
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB248831;  AB248832;  AB248833;  AB248834;  AB248835;  AB248836;  AB248837;  AB248838;  AB248839;  AB248840;  AB248841;  AB248842;  AB248843;  AB248844;  AB248845;  AB248846
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MeSH Terms
Descriptor/Qualifier:
Caliciviridae Infections / diagnosis*
DNA Primers / genetics
Feces / virology
Gastroenteritis / virology*
Humans
Molecular Diagnostic Techniques*
Molecular Sequence Data
Norovirus / classification,  genetics,  isolation & purification*
Nucleic Acid Amplification Techniques / methods*
RNA, Viral / analysis,  genetics
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/DNA Primers; 0/RNA, Viral
Comments/Corrections
Erratum In:
J Med Virol. 2007 Jun;79(6):863

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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