Document Detail


Evaluation of the applicability of backscattered light measurements to the determination of microbial cell densities in microtiter plates.
MedLine Citation:
PMID:  17061075     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The turbidity of a microbial suspension sample is routinely determined by measuring the optical density (often referred to as the "absorbance"). This method requires a dilution step at moderate and high cell densities in order to ensure that measurements fall within the region where biomass concentration is linearly correlated to optical density. The measurement of backscattered light (often referred to as the "reflectance"), which has so far been mainly applied to large-scale stirred tanks, should also be applicable on the microscale. To evaluate the validity of this assumption, a standard fluorescence microplate reader was adapted to measure backscattered light. Backscattered light readings from undiluted microbial fermentation samples determined using this modified reader gave similar growth curves to optical density measurements from diluted samples determined in a standard cell photometer. Indeed, the fact that the dilution procedure is not necessary for backscattered light measurements gives them an important advantage over optical density measurements. Such an apparatus is not only suitable for manual operation, but also shows the potential for integration into fully automated robotic systems used for high-throughput experimentation.
Authors:
Hartmut F Zimmermann; Thomas Raebiger
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Publication Detail:
Type:  Journal Article     Date:  2006-10-24
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  386     ISSN:  1618-2642     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-20     Completed Date:  2007-01-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2245-7     Citation Subset:  IM    
Affiliation:
Degussa AG, Rodenbacher Chaussee 4, 63457 Hanau-Wolfgang, Germany. hartmut.zimmermann@degussa.com
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MeSH Terms
Descriptor/Qualifier:
Bioreactors / microbiology*
Cell Proliferation
Corynebacterium glutamicum / cytology
Escherichia coli / cytology
Light*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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