| Evaluation of apoptosis in the liver preserved by simple hypothermia using histidine-tryptophan-ketoglutarate and prolactin-modified histidine-tryptophan-ketoglutarate solution. | |
| | |
MedLine Citation:
|
PMID: 21996184 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
INTRODUCTION: Organ ischemia is accompanied by cell death due to apoptosis. It occurs together with necrosis, which has more unfavorable consequences due to the release of cytokines that activate the inflammatory response cascade. The aim of this study was to assess the degree of apoptosis in porcine livers preserved by simple hypothermia for 12 hours using standard solutions (University of Wisconsin [UW] and histidine-tryptophan-glutarate [HTK]), and to evaluate the effect of prolactin (PRL) addition to the HTK solution. MATERIALS AND METHODS: The study was performed on the livers of Great White breed pigs, after inducing 30 minutes of warm ischemia (WIT30), followed by 30 minutes of perfusion-cooling to 4°C, and 12 hours of preservation. Livers were evaluated after preservation in Ringer's solution (control); UW (control reference fluid); HTK and HTK modified by the addition of prolactin (20 UI/L. Apoptosis was assessed in liver sections by the TdT-mediated dUTP nick-end labeling method after 12-hour preservation. We adopted a prevalence scale ranging from 0 to 3+, depending on the number of observed nuclei and apoptotic bodies (AB). RESULTS: Preservation in Ringer's solution yielded AB distribution at the 1+ level, with a lack of characteristic localization resulting from necrotic lesions. Analysis of the livers preserved in the UW solution showed high, 3+ level of AB presence. For the tested HTK solution, the observed ABs localization value was 3+, whereas in the PRL-modified group it was also 3+, but with a tendency to move from zone II to cluster III, which is important for liver metabolic functions. CONCLUSIONS: PRL improved the preservation properties of HTK for porcine livers by maintaining a high apoptosis level. It may stabilize cell membranes thus reducing the oncotic necrosis, promoting increased apoptosis during simple hypothermia. |
| | |
Authors:
|
G Budziński; R Wiaderkiewicz; A Caban; A Suszka-Świtek; G Oczkowicz; W Wystrychowski; M Heitzman; B Dolińska; F Ryszka; L Cierpka |
Related Documents
:
|
15658604 - Suppression of nuclear translocation of nuclear factor-kappab and nuclear factor of act... 8906804 - Human t cells require il-2 but not g1/s transition to acquire susceptibility to fas-med... 7835964 - Role of il-2 and il-4 in exacerbations of murine antigen-induced arthritis. 8964654 - Relationship of aging and cytokines to the immunomodulation by delta-9-tetrahydrocannab... 9262964 - Interleukin-10-mediated t cell apoptosis during the t helper type 2 cytokine response i... 10925264 - Distinct molecular mechanisms of fas resistance in murine b lymphoma cells. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Transplantation proceedings Volume: 43 ISSN: 1873-2623 ISO Abbreviation: Transplant. Proc. Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-10-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
|
Languages: eng Pagination: 2900-2 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of General, Vascular and Transplant Surgery, Medical University of Silesia, Katowice, Poland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of cysteine effect on redox potential of porcine liver preserved by simple hypothermia.
Next Document: Algorithm to Manage Highly Sensitized Kidney Transplant Recipients in Poland.