Document Detail


Evaluation of antioxidant system in normal semen
MedLine Citation:
PMID:  17569299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Reactive oxygen species (ROS) formation have the ability to alter reversibly or irreversibly the cellular function in humans. It has been proposed that the ROS alters the biochemistry and the physiology of the sperm. On the other hand, the antioxidative mechanisms could protect the sperms from the damage produced by free radicals. AIM: To determine the normal values for superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA) and nitric oxide (NOx) in the seminal liquid of healthy humans. PROCEDURES: Semen samples from 45 healthy men (22 to 47 years of age) were studied. The samples were obtained by masturbation and were collected in conical sterile tubes. Once centrifuged at 4 degrees C they were divided in aliquots to measure the concentration of SOD, GPx, MDA, and NOx. The analysis of the samples was realized in conformity with biochemical widely accepted methods. RESULTS: The concentrations of SOD and MDA both in the seminal liquid and in the spermatozoids were similar, SOD 0.43 +/- 0.09 U/mg prot. in the seminal liquid and 0.45 +/- 0.07 U/ mg prot. in spermatozoids, and MDA 0.33 +/- 0.07 nmoles/mg prot. and 0.37 +/- 0.10 nmoles/mg prot. in the seminal liquid and spermatozoids respectively. With regard to GPx it increased almost 13 times more in the spermatozoids (2547.77 +/- 48.59 U/mg prot.) than in the seminal liquid (197.54 +/-25.21 U/mg prot.). The NOx also increased lightly in the spermatozoids (4.45 +/- 0.43 Apmol) when compared with the seminal liquid (3.91 +/- 0.16 pumol). CONCLUSIONS: The measurement of the antioxidative and oxidative agents could serve to evaluate human infertility in those cases where the result of the spematobioscopy appears normal.
Authors:
Juan M Gallardo
Publication Detail:
Type:  English Abstract; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Revista de investigación clínica; organo del Hospital de Enfermedades de la Nutrición     Volume:  59     ISSN:  0034-8376     ISO Abbreviation:  Rev. Invest. Clin.     Publication Date:    2007 Jan-Feb
Date Detail:
Created Date:  2007-06-15     Completed Date:  2007-08-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421552     Medline TA:  Rev Invest Clin     Country:  Mexico    
Other Details:
Languages:  spa     Pagination:  42-7     Citation Subset:  IM    
Affiliation:
Unidad de Investigación Médica en Enfermedades Nefrológicas, UMAE-Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS. jmgallardo@salud.gob.mx
Vernacular Title:
Evaluación del sistema antioxidante en el semen normal.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antioxidants / analysis*
Glutathione Peroxidase / analysis*
Humans
Leukocyte Count
Lipid Peroxidation
Male
Malondialdehyde / analysis*
Middle Aged
Nitric Oxide / analysis*
Proteins / analysis
Reference Values
Semen / chemistry*,  cytology,  enzymology
Sperm Motility
Spermatozoa / chemistry*
Superoxide Dismutase / analysis*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Proteins; 10102-43-9/Nitric Oxide; 542-78-9/Malondialdehyde; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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