Document Detail


Evaluation of antioxidant status and oxidative stress in cattle naturally infected with Theileria annulata.
MedLine Citation:
PMID:  16901649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To assess the antioxidant status and oxidative stress in bovine theileriosis due to Theileria annulata blood samples were collected from 35 clinically affected cattle referred to Veterinary Teaching Hospital, School of Veterinary Medicine, Urmia University, Urmia, Iran. Complete blood count, piroplasm parasitemia percentage, erythrocyte glutathione peroxidase, superoxide dismutase, catalase and glucose-6-phosphate dehydrogenase activities, malondialdehyde concentration, osmotic fragility test and median corpuscular fragility were determined and the results were compared with those of 50 healthy controls. Of 35 affected cattle, 12 (34.28%) had severe anemia and 23 had mild to moderate anemia and parasitemia varied from 5 to 40%. The activities of erythrocyte glutathione peroxidase, superoxide dismutase and glucose-6-phosphate dehydrogenase were significantly lower (P<0.0001) and the activity of catalase was significantly higher in the affected cattle than in healthy ones (P<0.001). Malondialdehyde concentration in erythrocytes of affected cattle was significantly more than those of healthy cattle (P<0.001). The affected cattle showed increased fragility of erythrocytes, so that median corpuscular fragility (MCF) in affected group was significantly lower than those of healthy group (P<0.0001). Median corpuscular fragility showed a positive correlation with the severity of parasitemia (r=0.81, P<0.0005) and a negative correlation with the activities of GSH-Px (r=-0.78, P<0.0001), SOD (r=-0.71, P<0.0005), catalase (r=-0.53, P<0.018) and G6PD (r=-0.58, P<0.0005). The results of this study suggest that oxidative damage to RBCs may contribute to the pathogenesis of anemia in bovine theileriosis.
Authors:
S Asri Rezaei; B Dalir-Naghadeh
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Publication Detail:
Type:  Journal Article     Date:  2006-08-09
Journal Detail:
Title:  Veterinary parasitology     Volume:  142     ISSN:  0304-4017     ISO Abbreviation:  Vet. Parasitol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-23     Completed Date:  2006-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7602745     Medline TA:  Vet Parasitol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  179-86     Citation Subset:  IM    
Affiliation:
Department of Clinical Sciences, School of Veterinary Medicine, Urmia University, Urmia, P.O. Box 1177, Iran. s.asri@mail.urmia.ac.ir
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MeSH Terms
Descriptor/Qualifier:
Anemia / epidemiology,  veterinary
Animals
Case-Control Studies
Catalase / blood
Cattle
Erythrocytes / enzymology*,  metabolism
Female
Glucosephosphate Dehydrogenase / blood
Glutathione / blood
Glutathione Peroxidase / blood
Glutathione Transferase / blood
Iran / epidemiology
Lipid Peroxidation*
Male
Oxidative Stress* / physiology
Parasitemia / epidemiology,  veterinary
Superoxide Dismutase / blood
Theileria annulata / pathogenicity*
Theileriasis / enzymology*,  metabolism
Chemical
Reg. No./Substance:
70-18-8/Glutathione; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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