Document Detail


Evaluation of an antimicrobial ingredient prepared from a Lactobacillus acidophilus casein fermentate against Enterobacter sakazakii.
MedLine Citation:
PMID:  19350978     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previously two antimicrobial peptides, IKHQGLPQE (caseicin A) and VLNENLLR (caseicin B), were identified following the fermentation of sodium caseinate with the proteolytic strain Lactobacillus acidophilus DPC 6026. This study evaluated the ability of these peptides to kill Enterobacter sakazakii ATCC 12868 spiked in reconstituted infant formula. The survival of E. sakazakii populations in reconstituted infant formula containing a sodium caseinate fermentate was compared with survival in formula containing positive (monocaprylin) and negative controls. The L. acidophilus DPC 6026 sodium caseinate fermentate reduced pathogen numbers by >4 log CFU/ml at 37 degrees C, comparing favorably with the activity of monocaprylin. Additionally, E. sakazakii NCTC 8155 was inoculated into pasteurized, reconstituted infant formula (6 log CFUlml) followed by the addition of increasing concentrations of the fermentate (0.21 to 6.7% [wt/vol]). At a concentration of 0.21% (wt/vol), pathogen viability was maintained over 4 h at 6.0 log CFU/ml. In contrast, pathogen numbers increased approximately 100-fold in the control formula in the same time frame. At higher final fermentate concentrations (-3.33% [wt/vol]), numbers were reduced to 0 log CFU/ml over 60 min. The spectrum of activity of the fermentate against other foodborne pathogens was also determined and shown to be effective against Escherichia coli O157:H7 and Listeria innocua. Results indicate the potential of this fermentate as a built-in protection mechanism against E. sakazakii strains in reconstituted infant formula.
Authors:
M Hayes; E Barrett; R P Ross; G F Fitzgerald; C Hill; C Stanton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  72     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-04-08     Completed Date:  2009-04-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  340-6     Citation Subset:  IM    
Affiliation:
Teagasc Biotechnology Centre, Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Caseins / metabolism,  pharmacology*
Colony Count, Microbial
Dose-Response Relationship, Drug
Enterobacter sakazakii / drug effects*,  growth & development
Fermentation
Food Contamination / analysis*
Humans
Infant
Infant Food / microbiology*
Infant Formula
Infant, Newborn
Lactobacillus acidophilus / metabolism,  physiology*
Microbial Sensitivity Tests
Peptide Fragments / metabolism,  pharmacology
Chemical
Reg. No./Substance:
0/Caseins; 0/Peptide Fragments

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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