Document Detail

Evaluation of antibodies against hydatid cyst fluid antigens in the post-treatment follow-up of cystic echinococcosis patients.
MedLine Citation:
PMID:  19333201     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Cystic echinococcosis (CE), caused by the metacestode stage of Echinococcus granulosus, is one of the most important zoonoses worldwide. Long post-treatment follow-up is required because of possible relapse. The objective of this study was to determine the values of different antigenic fractions of sheep hydatid cyst fluid in the follow-up of CE patients after treatment. MATERIAL/METHODS: After gradient gel electrophoresis of sheep hydatid cyst fluid, 45 post-treatment (1 month -16 years) serum samples of CE patients treated with PAIR (puncture, aspiration, injection, and reaspiration) and five post-treatment (1 month) sera of operated CE patients were studied using Western blot. Twenty healthy individuals and five patients infected with other helminths served as controls. RESULTS: Antigens with molecular weights of 6.5-8, 14, 20, 29, 45, 50, 66, 116-120, 205, and 215 kDa were identified. The bands detected at the highest frequency were 29 kDa (10.4%), 45 kDa (17.2%), and 66 kDa (12.8%). Sera of the 20 healthy controls recognized no specific bands. All of the sera of the five patients with other parasitic diseases recognized one or more hydatid cyst fluid antigen. There was a statistically significant difference between the time after treatment and band weights (p<0.001). CONCLUSIONS: The protein bands of 29 and 205-215 kDa may be valuable in the follow-up of cystic echinococcosis patients as they disappear one year and six months, respectively, after therapy.
Tuncay Celik; Gülay Aral Akarsu; Ciğdem Güngör; Cemil Colak; Necati Ormeci
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical science monitor : international medical journal of experimental and clinical research     Volume:  15     ISSN:  1643-3750     ISO Abbreviation:  Med. Sci. Monit.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-31     Completed Date:  2009-05-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9609063     Medline TA:  Med Sci Monit     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  CR170-6     Citation Subset:  IM    
Department of Parasitology, Medical Faculty, Inonu University, Malatya, Turkey.
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MeSH Terms
Antibodies, Protozoan / immunology*
Antigens, Protozoan / immunology*
Blotting, Western
Case-Control Studies
Echinococcosis / drug therapy*,  immunology*
Electrophoresis, Polyacrylamide Gel
Reg. No./Substance:
0/Antibodies, Protozoan; 0/Antigens, Protozoan

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