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Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering.
MedLine Citation:
PMID:  20717710     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Alginate hydrogels in forms of discs and packed beds of microbeads (~800 μm) were tested in a novel bioreactor at 10% strain using two regimes: at a loading rate of 337.5 μm/s and at sequential increments of 50 μm displacement every 30 min. Compressive strength increased with the increase in alginate concentration (1.5 vs. 2% w/w) and the content of guluronic residues (38.5 vs. 67%). Packed beds of microbeads exhibited significantly higher (~1.5-3.4 fold) compression moduli than the respective discs indicating the effects of gel form and entrapped water. Short-term cultivation of microbeads with immobilized bovine calf chondrocytes (1.5% w/w, 33 × 10(6) cells/ml) under biomimetic conditions (dynamic compression: 1 h on/1 h off, 0.42 Hz, 10% strain) resulted in cell proliferation and bed compaction, so that the compression modulus slightly increased. Thus, the novel bioreactor demonstrated advantages in evaluation of biomaterial properties and cell-biomaterial interactions under in vivo-like settings.
Authors:
Jasmina Stojkovska; Branko Bugarski; Bojana Obradovic
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-18
Journal Detail:
Title:  Journal of materials science. Materials in medicine     Volume:  21     ISSN:  1573-4838     ISO Abbreviation:  J Mater Sci Mater Med     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9013087     Medline TA:  J Mater Sci Mater Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2869-79     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
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