Document Detail

Evaluation of the actin cytoskeleton state using an antibody-functionalized nanoneedle and an AFM.
MedLine Citation:
PMID:  22784496     Owner:  NLM     Status:  Publisher    
A cell diagnosis technique was developed, which uses an Atomic Force Microscope (AFM) and an ultra-thin AFM probe sharpened to a diameter of 200nm (nanoneedle). Due to the high aspect ratio of the nanoneedle, it was successfully inserted into a living cell without affecting its viability. Furthermore, by functionalizing the nanoneedle with specific antibodies and measuring the unbinding forces ('fishing forces') during evacuation of the nanoneedle from the cell, it was possible to measure specific mechanical interactions between the antibody-functionalized nanoneedle and the intracellular contents of the cell. In this study, an anti-actin-antibody-functionalized nanoneedle was used to evaluate the actin cytoskeleton state in living cells. To examine the effect of cytoskeleton condition on the measured fishing forces, the cytoskeleton-disrupting drugs cytochalasin D (cytD) and Y-27632 were used, showing a marked decrease in the measured fishing forces following incubation with either of the drugs. Furthermore, the technique was used to measure the time course changes in a single cell during incubation with cytD, showing a gradual time-dependent decrease in fishing forces. Even minute doses of the drugs, the effects of which were hardly evident by optical and fluorescence methods, could be clearly detected by the measurement of nanoneedle-protein fishing forces, pointing to the high sensitivity of this detection method. This technique may prove beneficial for the evaluation of cytoskeleton conditions in health and disease, and for the selection of specific cells according to their intracellular protein contents, without the need for introduction of marker proteins into the cell.
Yaron R Silberberg; Shingo Mieda; Yosuke Amemiya; Toshiya Sato; Takanori Kihara; Noriyuki Nakamura; Kyoko Fukazawa; Kazuhiko Ishihara; Jun Miyake; Chikashi Nakamura
Related Documents :
8101716 - Topical treatment of psoriatic plaques with 1,25-dihydroxyvitamin d3: a cell biological...
22512216 - Cassia alata leaf extract induces cytotoxicity in a549 lung cancer cells via a mechanis...
7067876 - Microenvironment of woringer-kolopp's disease.
9747656 - Quinolone photoallergy: photosensitivity dermatitis induced by systemic administration ...
15620416 - Morphological analysis of ghrelin and its receptor distribution in the rat pancreas.
24909296 - Targeting sim2-s decreases glioma cells invasion through mesenchymal-epithlial transition.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-29
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  -     ISSN:  1873-4235     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-7-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Continuous labeling of circulating tumor cells with microbeads using a vortex micromixer for highly ...
Next Document:  Novel implantable imaging system for enabling simultaneous multiplanar and multipoint analysis for f...