Document Detail


Evaluation of VIDAS Salmonella (SLM) immunoassay method with Rappaport-Vassiliadis (RV) medium for detection of Salmonella in foods: collaborative study.
MedLine Citation:
PMID:  15295882     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A collaborative study was conducted to compare the VIDAS Salmonella (SLM) with Rappaport-Vassiliadis (RV) method for detection of Salmonella in foods to the current standard method presented in the U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) and the culture method presented in AOAC's Official Methods of Analysis. The VIDAS SLM with RV method uses tetrathionate broth in combination with RV medium in place of selenite cystine broth for selective enrichment, thereby eliminating the hazardous waste issue for laboratories. Twenty five laboratories participated in the evaluation, each testing one or more of 8 test products: nonfat dry milk, dried egg, soy flour, lactic casein, milk chocolate, raw ground pork, raw ground turkey, and raw peeled shrimp. Results of the study showed no significant differences in the numbers of confirmed positive samples with the VIDAS SLM with RV procedure and the BAM/AOAC culture procedure. The VIDAS SLM with RV method was effective for rapid detection of Salmonella in foods. It is recommended that AOAC INTERNATIONAL modify the VIDAS Salmonella SLM procedure to include the RV method.
Authors:
Wendy A McMahon; Ann M Schultz; Ronald L Johnson
Publication Detail:
Type:  Journal Article; Validation Studies    
Journal Detail:
Title:  Journal of AOAC International     Volume:  87     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2004 Jul-Aug
Date Detail:
Created Date:  2004-08-05     Completed Date:  2004-10-15     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  867-83     Citation Subset:  IM    
Affiliation:
Silliker, Inc., Research Center, South Holland, IL 60473, USA. wendy.mcmahon@silliker.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cacao / microbiology
Culture Media
Dairy Products / microbiology
Eggs / microbiology
Flour / microbiology
Food Microbiology*
Immunoassay
Indicators and Reagents
Laboratories
Meat / microbiology
Penaeidae / microbiology
Salmonella / chemistry*
Shellfish / microbiology
United States
United States Food and Drug Administration
Chemical
Reg. No./Substance:
0/Culture Media; 0/Indicators and Reagents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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