Document Detail

Evaluation of T-cell activation in the duodenum of dogs with cutaneous food hypersensitivity.
MedLine Citation:
PMID:  20367052     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine whether skin-related clinical signs in cutaneous food hypersensitivity (CFH) coincide with immune reactivity in the intestine in dogs. ANIMALS: 11 dogs with CFH without intestinal clinical signs and 8 healthy control dogs. PROCEDURES: After a provocation and elimination diet, the duodenal gene expression levels of Th1-, Th2- and Treg-related cytokines and transcription factors were investigated by means of quantitative PCR assay. The presence of CD3(+), CD8(+), CD4(+), CD1c(+), gammadelta T-cell receptor(+), and major histocompatibility complex II(+) cells in duodenal epithelium and lamina propria were determined. RESULTS: The expression of Th1-, Th2-, and Treg-related genes in dogs with CFH and healthy control dogs was similar. Although clinical signs disappeared, there was no effect of the elimination diet on cytokines, transcription factors, or cellular phenotypes. CONCLUSIONS AND CLINICAL RELEVANCE: No change in T-cell phenotypes or a distinct Th1, Th2, or Treg profile was detected in the duodenum of dogs with only cutaneous clinical signs of food hypersensitivity. This suggested that the intestinal mucosa is not the primary site of T-cell activation that eventually leads to cutaneous food hypersensitivity.
Eveline Z Veenhof; Victor P Rutten; Ronald van Noort; Edward F Knol; Ton Willemse
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of veterinary research     Volume:  71     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-06     Completed Date:  2010-05-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  441-6     Citation Subset:  IM    
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands.
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MeSH Terms
Cytokines / genetics,  metabolism
Dermatitis, Allergic Contact / metabolism,  veterinary*
Dog Diseases / metabolism*
Duodenum / physiology*
Food Hypersensitivity / metabolism,  veterinary*
Gene Expression Regulation
T-Lymphocytes / metabolism*
Transcription Factors / genetics,  metabolism
Reg. No./Substance:
0/Cytokines; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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