Document Detail


Evaluation of the Single Yeast Cell's Adhesion toITO Substrates With Various Surface Energies via ESEM Nanorobotic ManipulationSystem.
MedLine Citation:
PMID:  22249767     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cell-surface adhesion force is important for cell activities and the development of bio materials. In this paper, a method for in-situ single cell (W303) adhesion force measurement was proposed based on nanorobotic manipulation system inside an environment scanning electron microscope (ESEM). An end effector was fabricated from a commercial atomic force microscope (AFM) cantilever by focused ion beam (FIB) etching. The spring constant of it was calibrated by nano-manipulation approach. Three kinds of hydrophilic and hydrophobic ITO plates were prepared by using VUV-irradiation and OTS coating techniques. The shear adhesion strength of the single yeast cell to each substrate was measured based on the deflection of the end effector. The results demonstrated that the cell adhesion force was larger under the wet condition in the ESEM environment than in the aqueous condition. It also showed that the cell adhesion force to hydrophilic surface was larger than that to the hydrophobic surface. Studies of single cells adhesion on various plate surfaces and environments could give new insights into the tissue engineering and biological field.
Authors:
Y Shen; M Ahmad; N Masahiro; K Seiji; H Michio; F Toshio
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-09
Journal Detail:
Title:  IEEE transactions on nanobioscience     Volume:  -     ISSN:  1558-2639     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101152869     Medline TA:  IEEE Trans Nanobioscience     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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